雪上一枝蒿总生物碱微乳的研制与质量评价  

Preparation and Quality Evaluation of Shortstalk Monkshood Root Total Alkaloids Microemulsion

作　　者：吴玉梅[1] 陈晓兰[1] 林亚平[2] 刘文[1] 唐红艳[1] 戴雅洁 

机构地区：[1]贵阳中医学院药学院,贵阳550002 [2]贵州省食品药品监督管理局,贵阳550003

出　　处：《中国实验方剂学杂志》2015年第23期7-11,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金地区基金项目(81260646);贵州省科学技术基金项目(黔科合J字[2012]2066号);贵州省中医药管理局中医药;民族医药科学技术研究课题(QZYY2011-58);贵阳中医学院博士基金项目(ZYY2011005)

摘　　要：目的:制备质量稳定的雪上一枝蒿总生物碱微乳,为该制剂的深入研究与开发提供参考。方法:选用不同的油相、乳化剂及助乳化剂,采用伪三元相图法进行雪上一枝蒿总碱微乳的处方筛选与制备,考察该制剂的稳定性及理化性质。采用HPLC测定雪上一枝蒿甲素的含量,流动相乙腈-p H 7.3磷酸盐缓冲溶液(20∶80),检测波长199 nm。结果:雪上一枝蒿总生物碱微乳最佳配比为雪上一枝蒿总生物碱、肉豆蔻酸异丙酯、聚氧乙烯35蓖麻油、无水乙醇、水质量分数分别为2%,3.3%,19.8%,9.9%,65.0%。载药微乳澄清透明,呈圆球形,粒径均在10~100 nm。于4,25℃下放置6个月内质量稳定。雪上一枝蒿甲素的质量分数2.28 mg·g^(-1)。结论:优化的雪上一枝蒿总生物碱微乳处方达到了预期的要求,为该制剂的后期研究提供实验基础。Objective： To prepare shortstalk monkshood root total alkaloids microemulsion. Method：Formulation of shortstalk monkshood root total alkaloids microemulsion was selected and prepared by pseudo-ternary phase diagram method with different oil phase,emulsifier and co-emulsifier,and preliminarily study its stability and physicochemical properties. HPLC was adopted to determine the content of bullatine A. Result： The best prescription was total alkaloids in Aconitum brachypodum of 2%,isopropyl myristate of 3. 3%,polyoxylethylene castor oil ether 35 of 19. 8%, absolute ethanol of 9. 9%, water of 65. 0%. The microemulsion was clear,transparent and spherical with particle size among 10-100 nm. Storage at 4 ℃ and 25 ℃ for six months is stable.The content of bullatine A was 2. 28 mg·g^-1. Conclusion： The optimized microemulsion formulation can achieve expected requirements. It can lay a foundation for further study of shortstalk monkshood root total alkaloids microemulsion.

关 键 词：雪上一枝蒿 总生物碱 微乳 粒径 伪三元相图 雪上一枝蒿甲素 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]