津力达颗粒联合通心络胶囊对2型糖尿病大鼠胰腺组织自噬的影响  

Effects of Jinlida Particles and Tongxinluo Capsule on Pancreas Autophagy in Type 2 Diabetes Mellitus Mice

作　　者：孙颖[1] 金鑫[2] 郭勇英[3,4] 刘红利[1,2] 崔雯雯[2] 魏聪 

机构地区：[1]南京中医药大学,南京210046 [2]黑龙江中医药大学,哈尔滨150000 [3]河北医科大学,石家庄050017 [4]河北以岭医药研究院,石家庄050035 [5]国家中医药管理局重点研究室(心脑血管络病),石家庄050035 [6]河北省络病重点实验室,石家庄050035

出　　处：《中国实验方剂学杂志》2015年第23期92-96,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB518606);河北省自然科学基金项目(H2013106139)

摘　　要：目的:观察中药复方津力达颗粒联合通心络胶囊对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠胰岛β细胞超微结构损伤情况、胰岛β细胞功能及胰腺组织自噬相关蛋白Beclin-1,Atg7,p62表达的影响。方法:采用高脂喂养结合小剂量链脲佐菌素(streptozotocin,STZ)30 mg·kg-1ip建立T2DM大鼠模型,15只雄性SD大鼠设为正常组正常饲料喂养,75只雄性SD大鼠高脂饲料喂养6周后STZ腹腔注射,根据血糖随机分为:模型组,津力达组(JLD),通心络胶囊组(TXL),联合用药组(JT),吡格列酮组(PLZ),JLD组于模型成功后给予津力达颗粒(3.0 g·kg-1·d-1),TXL组于模型成功后给予通心络胶囊(0.4 g·kg-1·d-1),JT组于模型成功后给予津力达颗粒(3.0 g·kg-1·d-1)加通心络胶囊(0.4 g·kg-1·d-1),PLZ组于模型成功后给予吡格列酮(10 mg·kg-1·d-1),连续ig 6周后,检测空腹血糖及血清胰岛素以计算胰岛β细胞功能指数,电镜观察各组大鼠胰岛β细胞超微结构;Western blot方法检测胰腺组织Beclin-1,Atg7,p62蛋白的表达变化。结果:与正常组相比较,模型组大鼠胰岛β细胞损伤减轻,胰岛β细胞功能指数明显降低,胰腺组织Beclin-1,Atg7蛋白表达明显减少,p62蛋白表达明显增加(P<0.01);与模型组比较,各给药组大鼠胰岛功能指数升高,Beclin-1,Atg7表达升高,p62蛋白表达减少(P<0.01),与JLD,TXL组比较,JT组Beclin-1,Atg7表达升高,p62蛋白表达减少(P<0.05)。结论:津力达颗粒联合通心络胶囊可以提高胰岛β细胞功能指数,其作用可能与促进胰岛自噬有关,而维持胰腺组织自噬水平可能是延缓糖尿病进展的有效措施。Objective： To observe the effect of traditional Chinese herbal compound Jinlida particles（ JLD） combined Tongxinluo capsule（ TXL） on the ultrastructure and function of islet β-cells and the protein expressions of Beclin-1,Atg7 and p62 of pancreasin diabetic in type 2 diabetes mellitus（ T2DM） mice. Method：T2DM mice were established by 6 weeks of high-fat diet combined with intraperitoneal injection of streptozotocin（ STZ）. Fifteen healthy male mice were set as the normal group. Another 75 T2 DM mice were randomly divided into 5 groups： the model group,the JLD group（ 3. 0 mg·kg^-1·d^-1）,the TXL group（ 0. 4 g·kg^-1·d^-1）,the combination group（ JT,3. 0 g·kg^-1·d^-1＋ 0. 4 g·kg^-1·d^-1） and the pioglitazone（ PLZ） group（ 10 mg·kg^-1·d^-1）. At the 6th week,levels of serum glucose and insulin of each group were measured and the index of β-cell function in HOMA（ HOMA-β） was calculated. The organelles injury of islet β-cells was investigated by transmission electron microscopy. Beclin-1,Atg7,p62 proteins were detected by Western blotting. Result：Compared with the normal group,the injury on islet β-cells was slight,the index of β-cell function in HOMA-β was decreased,the protein expressions of Beclin-1 and Atg7 decreased and the p62 protein expression increased in the model group（ P〈0. 01）. Compared with model group,the index of β-cell function in HOMA-β was increased,the protein expressions of Beclin-1 and Atg7 increased and the p62 protein expression decreased in the treatment groups（ P〈 0. 01）. Moreover,the results were better in the JT group（ P 〈0. 05）. Conclusion： JLD and TXL could improve the islet β-cells function,the effect may be related to inducing islet autophagy and maintaining the pancreas level of autophagy. The later may be the crucial measures on the time delay of diabetes progression.

关 键 词：津力达颗粒 通心络胶囊 胰岛β细胞 胰腺 自噬 BECLIN-1 Atg7 P62 

分 类 号：R285.5[医药卫生—中药学]