黄芪注射剂的制备及质量控制研究  

Preparation and Quality Control of Astragalus injection

作　　者：饶晓玲[1] 谢虹虹[2] 廖卫国[3] 

机构地区：[1]深圳市眼科医院深圳市眼科学重点实验室暨南大学附属深圳市眼科医院,深圳518001 [2]中山大学附属第三医院药剂科,广州510630 [3]广州中医药大学中药学院,广州510006

出　　处：《北方药学》2015年第12期84-86,共3页Journal of North Pharmacy

摘　　要：目的:筛选制备黄芪注射剂的最佳工艺。方法:利用动态吸附-解吸附方法,以紫外分光光度法测定黄芪总皂苷的含量,研究D-101大孔树脂分离纯化黄芪总皂苷的条件。结果:D-101大孔树脂分离纯化黄芪总皂苷的最佳条件为:采用浓度为含生药0.06g/m L黄芪提取液,最大上样量为1BV,流速为3BV/h,解吸附时用4BV的70%乙醇解吸附,收集70%乙醇洗脱液,得黄芪总皂苷提取物。结论:该工艺简单可行,能满足大量制备的需求。Objective： filtrate the best manufacture proeess of Astragali Radix injection. Methods： On the basis of the Dynamic adsorption-desovption method,using the UV-Vis to measure how much the total saponins of Astragalus it contains,researching the conditions of separating and purifying the total saponins of Astragalus by the means of D-101 macro resin. Results： The best conditions for using D- 101 macro resin to separate and purify the total saponins of Astragalus are as follow：the crude herbal dose of the Astragalus extract is at 0.06g/ml, the loading quantity of sample with its flow at 3BV/h is 1BV, and use 4BV 70% ethyl alcohol to do elution.Collecting the eluent, and then the total saponins of Astragulus extract is done.The rate of the total saponins of Astragulus collection can reach 86.36%. Conclusion： The manufacture process is feasible, and it can satisfy the preparation in quantity.

关 键 词：D101大孔吸附树脂 黄芪总皂苷 提取工艺 

分 类 号：R284.2[医药卫生—中药学]