人离体红细胞ATP含量与死亡时间和环境温度的关系  

Correlation of ATP content in RBCs with both postmortem intervals and temperature of surroundings

作　　者：韩玮[1,2] 于明鑫[3] 唐晖[4] 李常兰 贺敏[2] 任素萍[2] 焦章平[4] 于群[2] 

机构地区：[1]北京市公安局丰台分局物证鉴定所,北京100072 [2]军事医学科学院野战输血研究所,北京100850 [3]北京十一学校,北京100850 [4]北京市公安局法医检验鉴定中心,北京100192

出　　处：《中国医药导报》2015年第35期8-11,共4页China Medical Herald

基　　金：公安部应用创新计划项目(2009YYCXBJSJ002)

摘　　要：目的研究通过红细胞ATP含量推断早期死亡时间的适用温度范围和时间范围。方法采集12名健康捐血者全血各40mL，分装后分别保存于15、20、25、30℃的恒温箱中，以4h间隔取血样，采用生物发光法检测ATP含量，各组进行统计学分析，推导线性回归方程，计算各时间点的偏离时间。结果4组平均偏离时间差距较大，15、20、25、30℃组平均偏离时间分别为4．80、2．04、1．77、5．71h，而30℃组随着统计时间段的缩短平均偏离时间从5．71h缩短至1．67h。结论红细胞ATP含量这一参数最佳适用温度范围为20．30℃，有效评估时间20℃为64h．25℃为40h．30℃为28h。Objective To estimate suitable temperature and time scales about postmortem interval （PMI） in early stage by calculating ATP of RBCs. Methods 40 mL blood collected from 12 healthy donors were stored at 15, 20, 25, 30℃ respectively. Technical parameter as ATP content in RBCs was tested every 4 hours using bioluminescence assay. By statistical analysis, the regression equations and deviations of time point were obtained. Results There was a large gap on mean time deviation among 4 groups. The mean time deviation was 4.80 h in the 15℃ group, 2.04 h in the 20℃, 1.77 h in the 25℃and 5.71 h in the 30℃. Meanwhile, the mean time deviation was reduced from 5.71 h to 1.67 h over time in the 30℃group. Conclusion The best suitable temperature scale of ATP content in RBCs is during 20-30℃. Effective time scale is 64 h at 20℃, 40 h at 25℃, 28 h at 30℃.

关 键 词：死亡时间 红细胞ATP含量 环境温度 

分 类 号：R446.11[医药卫生—诊断学]