外源性重组人碱性成纤维细胞生长因子对大鼠压疮深部组织损伤后肌肉修复的作用  

作　　者：谢浩煌[1] 张宏宇[2] 毛挺挺 王晓慧[1] 潘莹莹[2] 肖健[2] 姜丽萍[3] 

机构地区：[1]浙江温州医科大学护理学院,325035 [2]浙江温州医科大学药学院,325035 [3]海交通大学附属新华医院

出　　处：《中华烧伤杂志》2015年第6期439-445,共7页Chinese Journal of Burns

基　　金：国家自然科学基金（81372064）

摘　　要：目的探讨外源性重组人bEGF（rhbFGF）对大鼠压疮深部组织损伤（DTI）后肌肉修复的作用。方法将48只SD大鼠按随机数字表法分为正常对照组、损伤对照组、伤后4d组、伤后7d组、伤后14d组、伤后21d组，每组8只。正常对照组大鼠不做任何处理，后5组大鼠在两侧后肢股薄肌处建立压疮DTI模型。损伤对照组大鼠伤后不做任何处理；另4组大鼠在伤后即刻分别于左侧后肢股薄肌处皮下注射100μg／mL的rhbFGF0．1mL，右侧后肢股薄肌处注射等体积生理盐水（NS），隔天注射1次。损伤对照组大鼠于伤后即刻、正常对照组大鼠于相同时相点，另4组大鼠分别于伤后第4、7、14、21天切取两侧后肢股薄肌处肌肉组织。HE染色观察股薄肌组织形态，免疫荧光法检测成肌素表达，蛋白质印迹法检测肌肉结构蛋白肌球蛋白重链（MyHC）、磷酸化蛋白激酶B（Akt）、磷酸化哺乳动物雷帕霉索靶蛋白（mTOR）表达。对数据行单因素方差分析、LSD检验。结果（1）正常对照组大鼠股薄肌细胞胞核大小均匀、排列紧密、结构清晰，无明显的炎性细胞浸润；损伤对照组大鼠股薄肌出现肌细胞固缩、溶解断裂，结构紊乱的病理退化现象；伤后4d组、伤后7d组、伤后14d组、伤后21d组大鼠经rhbFGF处理的股薄肌细胞肿胀、炎性浸润、结构紊乱等病理损伤现象均较组内经NS处理的股薄肌减轻，肌细胞增殖数目增多。（2）正常对照组、损伤对照组大鼠股薄肌成肌素表达阳性细胞数分别为（28±17）、（424-28）个；伤后4d组、伤后7d组、伤后14d组大鼠经NS处理的股薄肌成肌素表达阳性细胞数分别为（100±50）、（196±87）、（460±110）个，经rhbFGF处理的股薄肌成肌素表达阳性细胞数分别为（174±34）、（717±182）、（613±122）个，各组大鼠经rhbFGF处理的股薄肌成肌素表达阳性细胞数明显多于组内经NS处理的�Objective To investigate the effect of exogenous recombinant human basic fibroblast growth factor (rhbFGF) on the healing of muscles in rats after deep tissue injury of pressure ulcers.Methods Forty-eight SD rats were randomly divided into normal control group, injury control group, post injury day (PID) 4 group, PID 7 group, PID 14 group, PID 21 group according to the random number table, with 8 rats in each group.The rats in normal control group did not receive any treatment, whereas the rats in the latter 5 groups were established the deep tissue injury of pressure ulcer model on both sides of the gracilis muscle on the hind limb.The rats in injury control group did not receive any treatment after injury, while the rats in the latter 4 groups were given subcutaneous injection of 0.1 mL rhbFGF to the left gracilis in a dosage of 100 μg/mL immediately after injury, and an equal volume of normal saline (NS) was injected to right gracilis, once every other day.The rats in injury control group were sacrificed immediately after injury, and the rats in normal control group were sacrificed at the same time point.The rats in the other 4 groups were sacrificed on PID 4, 7, 14, 21, and the gracilis muscles on both sides were harvested respectively.The morphology of the gracilis muscle was examined after HE staining.The expression of myogenin in the tissues was detected by immunofluorescence method.The levels of muscle structural proteins myosin heavy chain (MyHC), phosphorylated protein kinase B (Akt), and phosphorylated mammalian target of rapamycin (mTOR) were determined by Western blotting.Data were processed with one-way analysis of variance and LSD test.Results (1) In normal control group, the nuclei of graciles cells were in uniform size, and they were closely arranged with clear structure, and there were no significant infiltration of inflammatory cells.In injury control group, the nuclei of graciles cells showed signs of pyknosis, dissolution, fracture and structural disorder.Swelling of muscle cells, inflammati

关 键 词：肌肉发育 成纤维细胞生长因子2 肌球蛋白重链 压疮 深部组织损伤 蛋白激酶B 哺乳动物雷帕霉素靶蛋白 

分 类 号：R632.1[医药卫生—外科学]