Mapping Toll-Like Receptor Signaling Pathway Genes of Zhikong Scallop(Chlamys farreri) with FISH  

作　　者：ZHAO Bosong ZHAO Liang LIAO Huan CHENG Jie LIAN Shanshan LI Xuan HUANG Xiaoting BAO Zhenmin 

机构地区：[1]Key Laboratory of Marine Genetics and Breeding of Ministry of Education,College of Marine Life Sciences,Ocean University of China

出　　处：《Journal of Ocean University of China》2015年第6期1075-1081,共7页中国海洋大学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31270047);the National High Tech R&D Program(2012AA10A410);the National Basic Research Program of China(2010CB126402);the National Key Technology R&D Program of China(2011BAD45B01 and 2011BAD13B05)

摘　　要：Toll-like receptor(TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop(Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes(Cf TLR, Cf Myd88, Cf TRAF6, Cf NFκB, and Cf IκB) containing bacterial artificial chromosomes(BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species.Toll-like receptor (TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop (Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes (CfTLR, CfMyd88, CfTRAF6, CfNF kappa B, and CfI kappa B) containing bacterial artificial chromosomes (BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species.

关 键 词：IMMUNOGENETICS CHLAMYS FARRERI TLR signaling pathway FISH 

分 类 号：S917.4[农业科学—水产科学]