补骨脂抗氧化及抗肿瘤活性成分的研究  

Study on antioxidant and antitumor active ingredient from Psoralea corylifolia

作　　者：聂丽娟[1] 李红梅[1] 郭星[1] 吴成柱[1] 张玉心[2] 霍强[1] 马涛[1] 

机构地区：[1]蚌埠医学院药学系,安徽蚌埠233030 [2]蚌埠医学院检验系,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2015年第11期1461-1464,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金项目(81302671);安徽省自然科学基金项目(1408085QH162);安徽省高等学校省级优秀青年人才基金项目(2013SQRL048ZD)

摘　　要：目的：研究补骨脂成分的抗氧化和抗肿瘤活性。方法：运用多种柱色谱等现代分离手段对补骨脂甲醇浸膏进行分离,利用磁共振技术和电喷雾电离质谱鉴定化合物的结构,并评价化合物的抗氧化和抗肿瘤活性。结果：分离出11个化合物,鉴定为：1补骨脂素,2异补骨脂素,3补骨脂定,4补骨脂苷,5异补骨脂苷,6双补骨脂酚A,7双补骨脂酚B,812,13-二氢-12,13-环氧补骨树脂酚;9补骨脂宁,10异补骨脂二氢黄酮,11补骨脂二氢黄酮甲醚。化合物3,6,7具有清除1,1-二苯基-2-三硝基苯肼自由基的能力;3,9~11对肝癌Hep G2细胞和乳腺癌MDA-MB-231细胞具有抗增殖作用,7对肝癌Hep G2细胞有一定抗增殖作用。结论：化合物3,6,7具有一定的抗氧化活性;3,7,9~（11）瑡具有一定的抗肿瘤活性。Objective： To screen the anti-oxidative and antitumor compounds from Psoralea corylifolia L. seeds. Methods： The methanol extract of Psoralea corylifolia L. was separated by a variety of column chromatography and the modern separation methods,the compounds were identified by nuclear magnetic resonance and electrospray ionization-mass spectrum. Results： Eleven compounds were obtained and identified as 1 psoralen,2 isopsoralen,3 psoralidin,4 psoralenoside,5 isopsoralenoside; 6 bisbakuchiols A,7bisbakuchiols B,8 12,13- dihydro- 12,913-epoxybabakuchiol; corylin,10 isobavachin,11 and bavachinin. Compounds 3,6,7shown scavenging effect to the 1,1-Diphenyl-2-picrylhydrazyl radical; compounds 3,9-11displayed the antiproliferation effect to the liver cancer Hep G2 and breast cancer MDA-MB-231 cells,compounds 7 displayed the antiproliferation effect to the liver cancer Hep G2 cells. Conclusions： Compounds 3,6,7 shown antioxidant activity; compounds 3,7,9-11 shown antitumor activity.

关 键 词：补骨脂 抗氧化 抗肿瘤 

分 类 号：R282.71[医药卫生—中药学]