γ-干扰素干预对乳鼠感染轮状病毒后肝损伤程度的影响  

Effect of γ- interferon on the injury degree of liver in suckling mouse with rotavirus infection

作　　者：杨梅[1] 陈兰举[1] 

机构地区：[1]蚌埠医学院第一附属医院儿科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2015年第11期1473-1476,共4页Journal of Bengbu Medical College

摘　　要：目的:检测肝脏核因子(NF)-κB的表达,评估γ-干扰素(IFN-γ)对乳鼠感染轮状病毒(RV)后的作用。方法:将乳鼠分为肠道外感染对照组、IFN-γ大小剂量干预组。腹腔注射RV建立2日龄RV肠道外感染模型。用大小剂量(2μg,0.2μg)IFN-γ在BALB/C乳鼠背部皮下注射3 d。模型建立后第5天取材,观察肝脏病理组织学变化,蛋白质印迹法和免疫组织化学法检测肝脏NF-κB表达。结果:IFN-γ小剂量干预组乳鼠肝脏病理损伤评分较肠道外感染对照组差异无统计学意义(P>0.05)。IFN-γ大剂量干预组乳鼠肝脏病理损伤评分均较肠道外感染对照组和IFN-γ小剂量干预组明显增高(P<0.01)。蛋白质印迹法和免疫组织化学法显示IFN-γ大小剂量干预组NF-κB表达随IFN-γ剂量的增加而增加(P<0.01)。结论:IFN-γ能刺激NF-κB的表达。肝脏的病理损伤与NF-κB在肝脏的表达量相关。Objective： To explore the expression of hepatic NF-κB for evaluating the effects of IFN-γ on suckling mouse with rotavirus（ RV） infection. Methods： The suckling mouse were divided into the control group,high and low-dose IFN-γ groups. The extraintestinal rotavirus infection model of 2-day-old suckling mouse was established by intraperitoneal injection of rotavirus. The 2 μg and 0. 2 μg IFN-γ were injected subcutaneously into the back of BALB / C suckling mouse for 3 days. The pathological changes and expressions of NF-κB detected by Western blot and immunohistochemistry in liver were observed after 5 days of model establishment. Results： The difference of the score of liver pathological damage between low-dose IFN-γ group and control group was not statistically significant（ P〈0. 05）. The score of liver pathological damage in high-dose IFN-γ group was significantly higher than that in control group and low-dose IFN-γ group（ P〈0. 01）. Western blotting and immunohistochemistry results showed that the expression of NF-κB increased with the dose increasing of IFN-γ（ P〈0. 01）. Conclusions： IFN-γ can stimulate the expression of NF-κB. The pathological damage of the liver was correlated with the expression of NF-κB.

关 键 词：轮状病毒 核因子κB Γ-干扰素 肠道外感染 BALB/C乳鼠 蛋白质印迹法 免疫组织化学 

分 类 号：R373.2[医药卫生—病原生物学]