全自动血沉仪采用乙二胺四乙酸抗凝血检测的临床应用分析  

Analysis on clinical application of full-automatic erythrocyte sedimentation rate analyzer in ditecting EDTA-anticoagulated blood

作　　者：张建平 马丽 马艳侠[3] 

机构地区：[1]咸阳市中心医院医学检验科,陕西咸阳712000 [2]咸阳市渭城区计划生育服务站,陕西咸阳712000 [3]陕西中医药大学附属医院检验科,陕西咸阳712000

出　　处：《国际检验医学杂志》2015年第23期3418-3420,共3页International Journal of Laboratory Medicine

摘　　要：目的采用乙二胺四乙酸(EDTA)抗凝全血的血沉仪同传统魏氏法及采用枸橼酸钠抗凝血的血沉仪红细胞沉降率(ESR)测定结果进行比较,以选择适合临床应用的自动化血沉仪。方法选择40例研究对象,分别采用传统的魏氏法、枸橼酸钠抗凝血的XC-A30自动血沉分析仪(以下简称XC血沉仪法)、EDTA抗凝血的VISION-C全自动动态血沉仪(以下简称VISION血沉仪法)测定ESR,将3种方法测定的ESR结果进行统计分析,并对VISION血沉仪法进行重复性试验。结果 3种方法测得ESR结果比较,差异有统计学意义(F=86.497,P=0.000)。XC血沉仪法与魏氏法ESR检测结果比较,差异有统计学意义(t=-4.08,P=0.004);VISION血沉仪法与魏氏法ESR检测结果呈正相关(r=0.975,P=0.000),二者检测结果比较,差异无统计学意义(t=0.65,P=0.638)。VISION血沉仪法两个水平质控品的重复性试验显示,批内及日间变异系数(CV)均低于10%。结论 VISION血沉仪法与魏氏法测得ESR结果相关性好,且操作简便、结果可靠,适合临床推广应用。Objective To compare the efficacies of automatic erythrocyte sedimentation rate（ESR）analyzer using EDTA as blood anticoagulant,traditional Westergren method and ESR analyzer using sodium citrate as blood anticoagulant in detecting ESR,in order to chose the appropriate one for clinical application.Methods A total of 40 samples were collected and divided into 3 groups.The ESR was detected by using XC-A30 automatic ESR analyzer using EDTA as blood anticoagulant（XC ESR analyzer）,Westergren method and VISION-C automatic ESR analyzer using sodium citrate as blood anticoagulant（VISION ESR analyzer）,respectively.Statistical analysis was used to comparing the detection results of ESR among the three methods.The repeatability test was carried out for evaluating repeatability of VISION ESR analyser.Results There was statistically significant difference in ESR detected by using Westergren,XC ESR analyzer and VISION ESR analyzer（F=86.497,P=0.000）.There was statistically significant difference in ESR between that detected by using XC ESR analyzer and that detected by using Westergren（t=-4.08,P=0.004）.The ESR detected by using VISION ESR analyzer was positively correlated with that detected by using Westergren（r=0.975,P=0.000）.There was no statistically significant difference in ESR between that detected by using VISION ESR analyzer and that detected by using Westergren（t=0.65,P=0.638）.The repetitive tests of analyzer shown that the within-run coefficient of variation（CV）and between-run CV both were less than 10%.Conclusion The ESR detected by using VISION ESR analyzer which is simple operation and with credible result has good correlation with that detected by using Westergren.The VISION ESR analyzer might be suitable for clinical application.

关 键 词：魏氏法 红细胞沉降率 临床应用 

分 类 号：R446.1[医药卫生—诊断学]