血清低密度脂蛋白胆固醇公式计算法与匀相测定法结果比较  

Result comparison between Martin formula calculation and homogenous direct measurement for the determination of serum LDL-C

作　　者：杨帆[1] 陈速[2] 刘志明[2] 

机构地区：[1]东南大学附属南京江北人民医院体检中心,210048 [2]东南大学附属南京江北人民医院检验科,210048

出　　处：《检验医学与临床》2015年第23期3510-3511,3514,共3页Laboratory Medicine and Clinic

摘　　要：目的对匀相直接测定法测定血清低密度脂蛋白胆固醇（LDL-C）与Martin公式计算法的结果进行对比观察。方法在Olympus AU 5800型全自动生化分析仪上对790份标本直接测定胆固醇、三酰甘油（TG）、高密度脂蛋白胆固醇、LDL-C,同时运用Martin公式计算出每份标本的LDL-C,按TG〈1.13 mmol/L、1.13-2.25mmol/L、〉2.25-3.39mmol/L、〉3.39-4.52mmol/L、〉4.52mmol/L分为5组进行统计分析。结果匀相直接测定法与公式法呈良好的正相关,除TG〉4.52 mmol/L组外,两种方法结果差异均有统计学意义（P〈0.05）。两种方法在LDL-C为3.37-4.14mmol/L,〉4.14mmol/L时表现为较好的符合率,在LDL-C〈3.37mmol/L时符合率较差。结论两种方法测定结果存在偏差,血清LDL-C的公式计算值高于匀相测定值,建议对于以治疗和监测心血管病为目的患者最好采用直接测定法。Objective To compare the results of low density lipoprotein cholesterol（LDL-C）detected by Martin formula calculation and homogenous direct measurement method.Methods TC,TG,LDL-C and HDL-C of 790 samples were determined directly by American Olympus AU5800 automatic biochemistry analyzer.LDL-C level of each sample was calculated by Martin formula at the same time.Samples were divided into five groups according to TG levels（〈1.13 mmol/L,1.13-2.25 mmol/L,〉2.25-3.39 mmol/L,〉3.39-4.52 mmol/L,and 〉4.52mmol/L）,and statistic analysis was performed among the groups.Results The results of homogenous direct measurement and Martin formula calculation showed a good positive correlation.The results of homogenous direct measurement were significantly different from those of Martin formula calculation among the other four groups except for TG〉4.52mmol/L group（P〈0.05）.The two methods showed high coincidence when the LDL-C level was 3.37-4.14mmol/L and 〉4.14mmol/L.When the LDL-C level was less than 3.37 mmol/L,the coincidence of the two method reduced.Conclusion The LDL-C result of Martin formula calculation is higher than that of homogenous direct measurement method,with an obvious bias.It suggests that homogenous direct measurement method is a better choice for the treatment or monitor in patients with cardiovascular disease.

关 键 词：低密度脂蛋白胆固醇 公式计算法 三酰甘油 胆固醇 

分 类 号：R446.11[医药卫生—诊断学]