表达Irkut病毒糖蛋白的重组复制缺陷型人5型腺病毒的构建与小鼠免疫试验  

Construction of a Recombinant Replication-defective Human Adenovirus Type 5 Expressing G Protein of Irkut Virus and the Immune Test in Mouse

作　　者：王玉莹[1,2] 陈奇 刘晔[2] 扈荣良[2] 张乐萃[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,长春130122 [3]北京军犬繁育训练基地疾病研究室,北京102101

出　　处：《病毒学报》2015年第6期634-640,共7页Chinese Journal of Virology

基　　金：国家"973"计划资助项目(2011CB500705)

摘　　要：本研究利用复制缺陷型人5型腺病毒载体,表达了我国蝙蝠狂犬病毒分离株IRKV-THChina12的G基因,并将重组病毒命名为rAd5-IRKV-G。Western blot和间接免疫荧光试验证明,IRKV-THChina12的G基因在重组腺病毒感染的293AD细胞中得到表达。将rAd5-IRKV-G对小鼠分别进行腹腔和肌肉免疫,同时设立wt-rAd5对照,结果显示两种免疫方式均能诱导小鼠产生抗IRKV中和抗体,且rAd5-IRKV-G组与wt-rAd5组差异显著(P≤0.05),腹腔免疫组与肌肉免疫组无显著差异(P>0.05)。结果表明,Irkut病毒糖蛋白可以作为疫苗抗原,而且构建的重组腺病毒具有较好的免疫原性。To develop a safe and effective new generation vaccine for IRKV-THChina12 prevention,we constructed a non-replicative recombinant human adenovirus carrying the IRKV-THChina12 Ggene,named as rAd5-IRKV-G.The IRKV-THChina12 G protein expressed by the recombinant human adenovirus in293 AD cells was detected by western blot and indirect immunofluorescence test.To evaluate the immunogenicity of the recombinant,mice were immunized with rAd5-IRKV-G by intramuscular（i.m.）or intraperitoneal（i.p.）route and with non-exogenous gene expressing wild type adenovirus wt-rAd5 as a control.Results showed that the rAd5-IRKV-G could induce continuous and statistically significant（P≤0.05）antiIRKV neutralizing antibody（NA）production in immunized mice by i.m.or i.p.route.In particular,no significant difference（P0.05）of the NA titers between the two administration routes were observed,that provides an alternative choice for animal immunization method in the future application.

关 键 词：狂犬病毒 蝙蝠 Irkut病毒 G基因 腺病毒 免疫 

分 类 号：S852.65[农业科学—基础兽医学]