肝素酶在内毒素血症致大鼠血管内皮糖萼破坏中的作用  

Role of heparanase in endotoxemia-induced destruction of vascular endothelial glycocalyx in rats

作　　者：蒋婷婷[1] 孙佳凤 夏江燕[1] 邱晓东[1] 袁静[1] 陆新建[1] 孙永瀛[2] 

机构地区：[1]东南大学附属中大医院麻醉科,南京市210009 [2]东南大学附属中大医院口腔科,南京市210009

出　　处：《中华麻醉学杂志》2015年第9期1131-1133,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金青年基金（6590000066）

摘　　要：目的 评价肝素酶在内毒素血症致大鼠血管内皮糖萼破坏中的作用.方法 SPF级健康雄性SD大鼠48只,8～10周龄,体重200～ 250 g,采用随机数字表法,将其分为3组（n=16）：对照组（C组）、脂多糖（LPS）组（L组）和LPS＋肝素组（LH组）.L组和LH组经股静脉注射LPS 15mg/kg制备内毒素血症模型,LH组同时静脉输注肝素钠注射液100 U· kg-1·h-1.于LPS注射后3、6h时,采集股静脉血样,测定血清硫酸肝素（HS）、多配体蛋白聚糖1抗体、E-选择素、细胞间粘附分子-1（ICAM-1）的浓度,取血样后处死取肺组织,HE染色后观察肺组织病理学改变,计算肺组织湿重/干重（W/D）比值.结果 与C组比较,L组血清HS、多配体蛋白聚糖1抗体、E-选择素、ICAM-1浓度和肺组织W/D比值升高,LH组血清HS、ICAM-1浓度升高（P＜0.05）.与L组比较,LH组血清HS、多配体蛋白聚糖1抗体、ICAM-1、E-选择素浓度和肺组织W/D比值降低（P＜0.05）.L组肺组织病理学损伤明显,LH组病理学损伤程度减轻.结论 肝素酶参与了内毒素血症致大鼠血管内皮糖萼破坏.Objective To evaluate the role of heparanase in endotoxemia-induced destruction of vascular endothelial glycocalyx in rats.Methods Forty-eight pathogen-free male Sprague-Dawley rats, aged 8-10 weeks, weighing 200-250 g, were randomly divided into 3 groups （n=16 each） using a random number table： control group （group C）, lipopolysaccharide （LPS） group （group L） and LPS＋ heparin group （group LH）.Endotoxemia was induced by intravenous LPS 15 mg/kg in L and LH groups, and heparin sodium 100 U · kg-1 · h-1 was infused simultaneously in group LH.At 3 and 6 h after LPS injection, blood samples were collected from the femoral vein for determination of the serum concentrations of serum heparin sulfate （HS）, syndecan-1, E-selectin and intercellular adhesion molecule-1 （ICAM-1） concentrations.The rats were then sacrificed, and lungs were removed for microscopic examination and for determination of wet/dry lung weight ratio （W/D ratio）.Results Compared with group C, the serum HS, syndecan-1, E-selectin and ICAM-1 concentrations and W/D ratio were significantly increased in group L, and the serum HS and ICAM-1 concentrations were increased in group LH （P〈0.05）.Compared with group L, the serum HS, syndecan-1, E-selectin and ICAM-1 concentrations and W/D ratio were significantly decreased in group LH （P〈0.05）.The pathological changes of lungs were obvious in group L, and were significantly mitigated in group LH.Conclusion Heparanase is involved in endotoxemiainduced destruction of vascular endothelial glycocalyx in rats.

关 键 词：肝素裂合酶 内毒素血症 多糖包被 内皮细胞 

分 类 号：R459.7[医药卫生—急诊医学]