盐酸戊乙奎醚对胸部创伤-失血性休克复苏致大鼠急性肺损伤时Fas/FasL表达的影响  

Effects of penehyclidine hydrochloride on Fas/FasL expression during acute lung injury induced by blunt chest trauma-hemorrhagic shock and resuscitation in rats

作　　者：吴晓静[1] 高文蔚[1] 冷燕[1] 赵博[1] 孟庆涛[1] 詹丽英[1] 夏中元[1] 

机构地区：[1]武汉大学人民医院麻醉科,430060

出　　处：《中华麻醉学杂志》2015年第9期1138-1141,共4页Chinese Journal of Anesthesiology

摘　　要：目的 探讨盐酸戊乙奎醚对胸部创伤-失血性休克复苏致大鼠急性肺损伤时Fas/FasL表达的影响.方法 SPF级健康雄性SD大鼠30只,体重245～ 275 g,8周龄,采用随机数字表法分为3组（n=10）：假手术组（Sham组）、胸部撞击-失血性休克复苏组（THSR组）和盐酸戊乙奎醚组（PHCD组）.建立胸部撞击-失血性休克复苏致急性肺损伤模型：将砝码（300 g）于95 cm高处自由落体,撞击大鼠心前区,5 min后经股动脉放血,使MAP在15 min内降至35～ 45 mmHg,并维持60min,然后进行复苏.PHCD组于失血性休克60 min时静脉注射盐酸戊乙奎醚2 mg/kg.于模型制备成功后6h放血处死大鼠,取肺组织,观察病理学结果,采用Western blot法和免疫组化SABC法检测Fas、FasL和caspase-8的表达,采用TUNEL法检测肺组织细胞凋亡指数（AI）,采用ELISA法测定肺组织IL-6和IL-1β含量.结果 与Sham组比较,THSR组和PHCD组肺组织Fas、FasL和caspase-8表达上调,AI升高,IL-6和IL-1β含量升高（P＜0.05）;与THSR组比较,PHCD组肺组织Fas、FasL和caspase-8表达下调,AI降低,IL-6和IL-1β含量降低（P＜0.05）.PHCD组肺组织病理学损伤较THSR组明显减轻.结论 盐酸戊乙奎醚抑制胸部创伤-失血性休克复苏诱发的大鼠肺组织细胞凋亡的机制与抑制Fas/FasL表达有关.Objective To investigate the effects of penehyclidine hydrochloride on Fas/FasL expression during acute lung injury induced by blunt chest trauma-hemorrhagic shock and resuscitation （HSR） in rats.Methods Thirty male SPF Sprague-Dawley rats, aged 8 weeks, weighing 245-275 g, were randomly assigned into 3 equal groups using a random number table： sham operation group （group Sham） , blunt chest trauma-HSR group （group THSR） and penehyclidine hydrochloric group （group PHCD）.The model of acute lung injury induced by blunt chest trauma-HSR was induced by dropping a 300 g weight onto a precordium in anesthetized rats.Blood was withdrawn via the femoral artery 5 min later until mean arterial pressure was decreased to 35-45 mmHg within 15 min, and maintained at this level for 60 min, followed by resuscitation.In PHCD group, PHCD 2 mg/kg was injected intravenously at 60 min after hemorrhagic shock.At 6 h after the model was established, the rats were sacrificed, the lungs were then removed for microscopic examination of pathologic changes and for determination of Fas, FasL and caspase-8 expression, and interleukin-6 （IL-6） and IL-1β contents in lung tissues.Apoptotic index was calculated.Results Compared with group Sham, the expression of Fas, FasL and caspase-8 was significantly up-regulated, and AI and contents of IL-6 and IL-1β were increased in THSR and PHCD groups （P〈O.05）.Compared with group THSR, the expression of Fas, FasL and caspase-8 was significantly down-regulated,and AI and contents of IL-6 and IL-1β were decreased in group PHCD （P〈0.05）.The pathologic changes of lungs were significantly reduced in group PHCD compared with group THSR.Conclusion The mechanism by which penehyclidine hydrochloride inhibits lung cell apoptosis induced by blunt chest trauma-HSR is associated with inhibition of Fas/FasL expression in rats.

关 键 词：胆碱能拮抗剂 胸部损伤 休克 出血性 呼吸窘迫综合征 成人 抗原 CD95 Fas配体蛋白质 Antigens CD95 

分 类 号：R614[医药卫生—麻醉学]