三黄片对糖尿病大鼠肾脏细胞间黏附分子1及血管细胞间黏附分子1表达的影响  

Influence of Sanhuang Tablets on Renal Expression of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in Diabetes Mellitus Rats

作　　者：田风胜[1] 李娟[1] 王霞 王佟[3] 崔荣岗[1] 边云[1] 苏阳[1] 

机构地区：[1]河北医科大学附属沧州中西医结合医院内分泌科,河北沧州061001 [2]沧州高等医学专科学校,河北沧州061001 [3]河北医科大学附属沧州中西医结合医院心血管内科,河北沧州061001

出　　处：《医学综述》2015年第23期4340-4343,共4页Medical Recapitulate

摘　　要：目的探讨三黄片对糖尿病大鼠肾脏细胞间黏附分子1(ICAM1)及血管细胞间黏附分子1(VCAM1)表达的影响。方法制备糖尿病大鼠模型,成模后,用简单随机抽样方法抽取20只分为模型组(10只)和三黄片组(10只),并设立正常组(10只)。三黄片组给予三黄片10 g/kg灌胃,其余两组每日给予同等剂量纯净水灌胃。8周后,取肾脏制备病理切片,链霉菌抗生物素蛋白-过氧化物酶连结法免疫组织化学染色,观察ICAM1及VCAM1的阳性表达。结果正常组大鼠肾质量和肾质量/体质量分别为(1.79±0.20)g和(3.81±0.49)×10^(-3),模型对照组大鼠肾质量和肾质量/体质量分别为(2.29±0.27)g和(8.35±0.68)×10^(-3),三黄片组大鼠肾质量和肾质量/体质量分别为(2.01±0.14)g和(6.08±0.69)×10^(-3)。模型对照组及三黄片组大鼠肾质量和肾质量/体质量显著高于正常组(P<0.05),三黄片组大鼠肾质量和肾质量/体质量显著低于模型对照组(P<0.05)。正常组大鼠尿素氮和肌酐为(7.09±1.03)mmol/L、(42±6)μmol/L,模型对照组大鼠尿素氮和肌酐分别为(10.14±1.13)mmol/L、(56±8)μmol/L,三黄片组大鼠尿素氮和肌酐为(9.33±1.08)mmol/L、(49±7)μmol/L。模型对照组和三黄片组大鼠较正常组大鼠尿素氮和肌酐显著升高(P<0.05)。结论三黄片能明显抑制糖尿病大鼠肾脏ICAM-1及VCAM1表达,具有保护糖尿病大鼠肾脏的作用。Objective To explore influence of Sanhuang tablets on renal expression of intercellular adhesion molecule 1 （ ICAM1 ） and vascular cellular adhesion molecule 1 （ VCAM-1 ） in diabetes mellitus （DM） rats. Methods The DM rat model was made. 20 rats were randomly selected and divided into model group（10 rats） and Sanhuang group（ 10 rats） ,and a normal control group（ 10 rats） was set up. Sanhuang tablets were given to the Sanhuang group, by 10 g/kg, gavage; the other two groups were given the same amount of pure water,gavage. 8 weeks later,kidneys were removed and the pathologic slices were prepared to observed the expression of ICAM-1 and VCAM-1 by streptavidin-perosidase（SP） immunohistochemical staining. Results Kidney weight and kidney weight/body weight ratio of the normal control group were（ 1.79 ±0.20） g,（3.81 ±0.49） ×10^-3,of the model group were （2.29 ±0.27） g,（8.35 ±0.68） ×10^-3,of Sanhuang group were （2.01 ±0. 14） g, （6. 08 ±0.69）×10^-3. Model group and Sanhuang group were significantly higher than the normal control group （ P 〈 0. 05 ）, and the Sanbuang group lower than the model group （ P 〈 0. 05 ）. Blood urea nitrogen and creatinine of the normal control group were （ 7.09 ±1.03 ） retool/L,（42±6）μmol/L,of the model group were （10. 14 ±1.13） mmol/L, （56 ±8） μmol/L,of the Sanhuang group were （9.33 ±1.08 ） mmol/L, （49 ±7 ） μmo]/L. The model group and Sanhuang group were significantly increased than the normal control group （ P 〈 0. 05 ）. Conclusion Sanhuang tablets can inhibit the expression of ICAM-1 and VCAM-1 and protect kidney of the diabetic rats.

关 键 词：糖尿病模型 三黄片 肾脏 

分 类 号：R589.1[医药卫生—内分泌]