Image-Pro Plus联合Photoshop图像分析软件能准确定量测定兔海马区微循环的改变  

Using Image-Pro Plus combined with Photoshop image analysis software for quantitative measurement of changes in hippocampus microcirculation

作　　者：黄陆平[1] 莫云长[1] 戴勤学[1] 金深辉[1] 王均炉[1] 

机构地区：[1]温州医科大学附属第一医院麻醉科,浙江温州325000

出　　处：《中国中西医结合急救杂志》2015年第6期587-590,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目（81273926）;浙江省自然科学基金资助项目（LY15H290006）

摘　　要：目的：探讨一种用图像分析软件Image-Pro Plus（IPP）联合图像处理软件Photoshop定量测定海马组织微循环改变的新方法。方法选择双侧颈总动脉结扎2周后无神经功能障碍的日本大耳白兔22只，按随机数字表法分为蛛网膜下腔出血（SAH）组和对照组，每组11只。采用枕大池二次注血法建立兔症状性脑血管痉挛模型；于第1次注血后7 d处死动物，进行大脑灌注固定，取兔海马区组织，用免疫组化法测定CD34。利用IPP 6.0联合Photoshop软件，定量测定海马组织中微血管密度（MVD）计数和统计场微血管面积比。结果 CD34可以有效标识微血管内皮细胞；用IPP 6.0可自动、准确地计算出海马区MVD和统计场微血管面积比。与对照组比较，SAH组兔海马区MVD计数与面积比均明显减少，差异均有统计学意义〔MVD（个/统计场）为3.87±0.67比5.17±0.53，统计场微血管面积比为（0.86±0.20）%比（1.40±0.17）%，均P＜0.05〕。结论 CD34可以用于标识微血管。IPP 6.0联合Photoshop图像分析软件是一种高效、准确统计组织中微血管数量与面积的工具，不仅操作简便，而且数据计算自动生成，可以准确反映兔症状性脑血管痉挛后海马区微循环改变。Objective To investigate a new method using Image-Pro Plus （IPP） combined with Photoshop image analysis software to quantitatively measure the changes in microcirculation in hippocampus.Methods Twenty-two Japanese white rabbits that had received bilateral carotid artery ligation for 2 weeks without neurological dysfunction were divided into a subarachnoid hemorrhage （SAH） group and a control group, 11 rabbits in each group. The rabbit model of symptomatic cerebral vasospasm was established by the method of twice injecting blood into occipital cistern. On the 7th day after the first time of injecting blood, the rabbits were sacrificed, cerebral perfusion fixation was carried out, the hippocampus was harvested, and CD34 was determined by immunohistochemical determination. IPP 6.0 combined with Photoshop image analysis software was used to quantitatively measure the count of hippocampal microvessels density （MVD） and the field （for statistics）/microvascular capillary area ratio was calculated.Results CD34 could effectively identify microvascular endothelial cells, and using IPP 6.0 could automatically and accurately calculate MVD and field （for statistics）/micrevascular area ratio in hippocampus. Compared with the control group, in SAH group, the MVD and the area ratio in hippocampus were significantly reduced, the differences being statistically significant [MVD （count/area）： 3.87±0.67 vs. 5.17±0.53, area ratio： （0.86±0.20）% vs. （1.40±0.17）%, bothP 〈 0.05].Conclusions CD34 can be used to identify microvessels, IPP 6.0 image analysis software co-Photoshop is a high efficient and accurate new method to measure the microvessels count and calculate the field/microvessel area ratio, not only it is easy to operate, but also the data can be automatically calculated and generated, reflecting precisely the changes in microcirculation in hippocampus after symptomatic cerebral vasospasm in rabbits.

关 键 词：Image-Pro Plus图像分析软件 Photoshop图像分析软件 脑血管痉挛 

分 类 号：R193[医药卫生—卫生事业管理]