四逆散对LPS诱导抑郁小鼠模型的抗抑郁作用及IDO调节机制  

Antidepressant effects and IDO regulatory mechanism of Sini Powder on depression mouse induced by LPS

作　　者：周静洋[1] 鲁艺[1] 李海娜[1] 张静[1] 畅洪昇[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中华中医药杂志》2015年第12期4431-4433,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81373584)~~

摘　　要：目的:研究四逆散对色氨酸(TRP)-犬尿氨酸(KYN)代谢途径及其关键代谢酶吲哚胺2,3过氧化酶(IDO)活性和含量的影响。方法:脂多糖(LPS)1mg/kg腹腔注射造成小鼠抑郁模型,采用ELISA方法检测血清γ干扰素(IFN-γ)含量,HPLC-MS/MS技术检测脑组织TRP、KYN含量,实时荧光定量PCR检测脑组织中IDO的m RNA表达水平。结果:与正常组比较,LPS注射4h后可以引起模型组小鼠不动时间延长,血清IFN-γ明显增高(P<0.05),同时,脑组织IDO活性、IDO m RNA表达增多(P<0.01)。与模型组比较,四逆散能缩短小鼠不动时间,降低血清IFN-γ含量(P<0.05),且能抑制IDO活性及m RNA表达数量(P<0.01)。结论:四逆散阻断了炎性因子诱导IDO激活途径,抑制了IDO的活性,并降低其含量,通过调节TRP-KYN代谢产生抗抑郁作用。Objective： To study the inl uence of Sini Powder on metabolic pathways of tryptophan-kynurenine（TRPKYN） and the activity and contents of the key metabolism enzyme as indoleamine 2, 3-dioxygenase（IDO）. Methods： The mouse model was established by using intraperitoneal injection with LPS（1mg/kg）. ELISA was used to detect the content of IFN-γ in serum. The contents of tryptophan and kynurenine in brain tissues were detected by using HPLC-MS/MS technique. The m RNA expression level of IDO in brain tissues was detected by using real-time PCR. Results： 4 hours after injecting with LPS, the immobility time of mice in model group was prolonged, the content of IFN-γ in mouse serum was increased significantly（P〈0.05）, meanwhile, the activity of IDO and the m RNA expression level of IDO in brain tissues were also increased（P〈0.01）. Sini Powder could shorten the immobility time of mice, reduce the content of IFN-γ in serum（P〈0.05）, and inhibit the activity and the m RNA expression of IDO（P〈0.01）. Conclusion： Sini Powder could block the activation pathway of IDO, inhibit the activity of IDO, reduce the content of it, and produce anti-depression effect by adjusting the TRP-KYN metabolism.

关 键 词：四逆散 脂多糖 抗抑郁 吲哚胺2 3过氧化酶 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]