白细胞介素-4诱导人支气管上皮细胞ORMDL3基因表达的时效性  

Time-effect of interleukin 4 on inducing the expression of ORMDL3 gene in human bronchial epithelial cells

作　　者：凌昊[1,2,3] 赵霞[1,2] 

机构地区：[1]南京中医药大学中医儿科研究所 [2]江苏省儿童呼吸疾病(中医药)重点实验室,南京210029 [3]宜兴市中医医院,无锡214200

出　　处：《中华中医药杂志》2015年第12期4438-4440,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173299);国家重点学科课题(No.EZK2012005);江苏省研究生培养创新工程项目课题(No.CXLX13-584)~~

摘　　要：目的:观察白细胞介素-4(IL-4)诱导人支气管上皮细胞(16HBE)不同时间血清类黏蛋白1样蛋白3(ORMDL3)的表达,探讨在细胞模型中IL-4对ORMDL3的转录和合成的影响。方法:用100ng/m L的IL-4诱导支气管上皮细胞后,分别在不同时间段(0、12、18、24、36、48h)用RT-PCR法检测ORMDL3 m RNA的表达,Western Blot法检测ORMDL3蛋白的表达。结果:正常支气管上皮细胞有微量的ORMDL3表达,IL-4诱导细胞后,ORMDL3m RNA和蛋白的表达均增加,在一定范围内呈时间依懒性,与对照组比较差异有统计学意义(P<0.05),其他各组与24h组比较差异有统计学意义(P<0.01)。结论:IL-4能诱导ORMDL3高表达,并且在一定范围内呈时间依赖性。Objective： To observe the expression of ORMDL3 in human bronchial epithelial cells（16 HBE） induced by interleukin 4（IL-4） at different time and explore the effect of IL-4 on the transcription and synthesis of ORMDL3 in cell model. Methods： IL-4（100ng/m L） was used to induce the bronchial epithelial cells. At 0, 12, 18, 24, 36 and 48 h after experiment, RT-PCR was used to determine the expression of ORMDL3 m RNA, and Western Blot was used to determine the expression of ORMDL3 protein. Results： There was few ORMDL3 in the normal bronchial epithelial cells, however, the expressions of ORMDL3 m RNA and protein were both increased by IL-4 that depended on the time in a certain extent. Furthermore, there was a statistical difference in the expression of ORMDL3 between the experimental groups and the control group（P〈0.05）. While, in all experimental groups, there were significant statistical differences in the expression of ORMDL3 between the 24 h experimental group and the others（P〈0.01）. Conclusion： IL-4 could induce an overexpression of ORMDL3, which had a time-effect relationship in a certain extent.

关 键 词：白细胞介素-4 人支气管上皮细胞 血清类黏蛋白1样蛋白3 时效 

分 类 号：R562.25[医药卫生—呼吸系统]