穿龙薯蓣皂苷调节再生障碍性贫血免疫共刺激分子的研究  

Study on dioscorea saponin regulating immune co-stimulatory molecule in aplastic anemia

作　　者：刘宝山[1] 马霖[2] 苗润静[3] 杨向东[3] 张蕾[3] 王兴丽[3] 纪超伦 

机构地区：[1]天津医科大学总医院,天津300052 [2]天津中医药大学研究生部,天津300193 [3]天津中医药大学第一附属医院,天津300193

出　　处：《中华中医药杂志》2015年第12期4441-4443,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173415);天津市应用基础与前沿技术研究计划(No.11JCYBJC12200)~~

摘　　要：目的:通过研究T细胞表达的共同刺激分子(CM)CD28、CD152、CD80、CD86在再生障碍性贫血(再障)小鼠的表达情况,探讨穿龙薯蓣皂苷治疗再障的作用机制。方法:用免疫介导法建立再障小鼠模型,随机分成模型组,高、中、低剂量组,雷公藤多苷组,环孢素组(Cs A组),并设正常组作为对照。造模第8天开始给药,正常组及模型组予等量0.9%氯化钠溶液。连续给药7d,眶丛静脉取血,对比外周血象,流式细胞术检测CD28、CD152、CD80、CD86的表达。结果:与正常组比较,模型组白细胞(WBC)、血红蛋白(HGB)及血小板(PLT)计数均显著减少(P<0.01),CD28、CD80、CD86的表达显著升高(P<0.05),CD152显著降低(P<0.05);与模型组比较,穿龙薯蓣皂苷各剂量组WBC、HGB及PLT计数均显著升高(P<0.05);中、高剂量组CD28、CD80、CD86的表达均显著减低,CD152的表达显著升高(P<0.05)。结论:穿龙薯蓣皂苷可能通过调节CM的表达从而发挥对再障的免疫调节作用。Objective： To study the expression of co-stimulatory molecule（CM） CD28, CD152, CD80, CD86 expressed in T-lymphocytes in mice aplastic anemia（AA） model, and to explore the mechanism of dioscorea saponin to treat the disease. Methods： Evaluating the models of AA mice were established by immune-mediated method. Randomly dividing mice into 7 groups： model group, high, middle groups, and low dose group, Tripterygium Wilfordii Polyglycoside group, Cs A group and normal group. From the eighth day of the experiment, the mice in the administration groups and normal group were fed with medicines and normal saline from the eighth day of the experiment. After continuous administration of 7d, blood samples from retro-orbital plexus were collected and detected, CD28, CD152, CD80 and CD86 were tested with flow cytometry. Results： Compared with normal group, WBC, HGB, PLT counts and CD152 in model group were significantly decreased（P〈0.01, P〈0.05）. CD28, CD80, CD86 in middle, high dose groups were significantly increased（P〈0.05）. Compared with the model group, WBC, HGB, PLT counts in high, middle, low dose groups were significantly increased（P〈0.05）, CD28, CD80, CD86 in middle, high dose groups were significanty decreased（P〈0.05）, CD152 in middle, high dose groups were significantly increased（P〈0.05）. Conclusion： It is possible that the expression of the CM maybe the immunology mechanism of the Dioscorea Saponin to treat AA.

关 键 词：穿龙薯蓣皂苷 再生障碍性贫血 共刺激分子 小鼠 

分 类 号：R285.5[医药卫生—中药学]