西花蓟马细胞色素P450基因cDNA片段克隆与mRNA表达分析  

Cloning and mRNA expression analysis of cDNA fragments of cytochrome P450 genes in Frankliniella occidentalis

作　　者：董红刚 谢志娟[2] 杜予州[3] 王建军[3] 

机构地区：[1]扬州市邗江区植保植检站,江苏扬州225009 [2]泰州市靖江市植保植检站,江苏泰州214500 [3]扬州大学园艺与植物保护学院,江苏扬州225009

出　　处：《环境昆虫学报》2015年第6期1188-1194,共7页Journal of Environmental Entomology

基　　金：公益性行业(农业)科研专项(200803025;201103026)

摘　　要：细胞色素P450介导的解毒作用增强是昆虫对杀虫剂产生抗性的重要机制。本文通过RT-PCR克隆了西花蓟马CYP4家族5个细胞色素P450基因c DNA片段。多重序列比对发现,这5个基因在氨基酸水平的一致性在40%-76%之间。采用实时荧光定量PCR对5个CYP4基因的mRNA表达水平的分析发现,阿维菌素抗性品系CYP4-1、CYP4-2和CYP4-5的表达水平分别是敏感品系的3.50、4.00和2.48倍,表明西花蓟马对阿维菌素的抗性可能与这3个CYP4基因的过量表达相关。Enhanced detoxification mediated by cytochrome P450 monoxygenases is a major mechanism of insect resistance to insecticides. In this study,five c DNA fragments of cytochrome P450 genes belonging to the CYP4 family were cloned by RT-PCR from the western flower thrips（ WFT）,Frankliniella occidentalis. An amino acid sequence alignment shows that these CYP4 genes shared amino acid identity ranging from 40% to 76%. The real-time q PCR analysis of mRNA expression levels of the five CYP4 genes revealed that the relative expression levels of CYP4-1,CYP4-2 and CYP4-5 genes in the abamectin resistant strain were 3. 50、4. 00 and 2. 48 times higher than that in the susceptible strain,respectively,suggesting the possible involvement of overexpression of these three CYP4 genes in the WFT resistance to abamectin.

关 键 词：西花蓟马 细胞色素P450 RT-PCR 实时荧光定量PCR 

分 类 号：Q965[生物学—昆虫学] S433.89[农业科学—农业昆虫与害虫防治]