1，25-二羟胆骨化醇对2型糖尿病大鼠肾损伤的影响  

Impact of 1, 25 dihydroxycholecalciferol on Kidney Injury of Type 2 Diabetic Rats

作　　者：邓娟娟[1] 张丹[1] 韩晴[1] 孔亚婷[1] 朱慧铭[1] 朱正庭[1] 叶明[1] 彭艳[1] 潘希峰[1] 李书国[1] 

机构地区：[1]三峡大学第一临床医学院宜昌市中心人民医院老年病科,湖北宜昌443003

出　　处：《医学临床研究》2015年第11期2101-2103,共3页Journal of Clinical Research

基　　金：宜昌市科技局课题（编号：A13301-08）

摘　　要：【目的】探讨1，25-二羟胆骨化醇（活性维生素D）对2型糖尿病大鼠肾损伤的影响。【方法】30只雄性Wistar大鼠随机分为普通饲料组即正常组（N组，10只）和高脂高糖饲料组（建模组，20只），2型糖尿病模型组建模成功后随机分为两组，每组10只，糖尿病组（M组）经腹腔注射2．5ug／（kg·d）花生油，D组腹腔注射2．5ug／（kg．d）溶于花生油中的1，25-二羟胆骨化醇。8用后留取大鼠血标本，检测血糖、血肌酐及尿素氮；同时处死大鼠留取肾组织，WesternBlot检测肾组织中肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）并比较。【结果】M组、D组大鼠血糖、尿素氮、肌酐均显著高于N组（P〈0．05），但D组血糖、尿素氮及肌酐较M组明显降低（P〈0．05）；M组、D组大鼠肾组织中TNF-α、TGF-β1水平显著高于N组，而D组较M组明显下调（P〈0．05）。【结论】1，25-二羟胆骨化醇可通过抑制糖尿病大鼠肾脏TNF-α、TGF-β1的表达，对糖尿病大鼠肾脏损伤起保护作用。[Objective]To study the impact of 1, 25 dihydroxycholecalciferol on kidney injury of type 2 diabetic rats. [Methodsl30 male Wistar rats were randomly divided into normal diet group （N group, 10 rats） and high fat and high glucose diet group （model group, 20 rats）, Type 2 diabetes model group were successfully set and randomly divided into two groups, with 10 rats in each group, diabetes mellitus group （M group） was treated by intraperitoneal injection of 2.5 g/（kg ·d） of peanut oil, and D group by intraperitoneal injection 2.5 μg/（kg·d） 1, 25 dihydroxy- cholecalciferol dissolved in peanut oil. After 8 weeks, blood samples were collected to detect blood glucose, creatinine and urea nitrogen;At the same time, the rats were sacrificed and the kidney tissues were kept.Tumor necrosis factor alpha （TNF-α）, transforming growth factor beta 1 （TGF-β1） were detected by Blot Western and compared with that in renal tissue.[Results]Blood glucose, urea nitrogen and creatinine in M group and D group were significantly higher than those in N group （ P〈0.05）, but the blood glucose, urea nitrogen and creatinine in D group were significantly lower than that in M group （ P 〈0.05）; The levels of TNF-α.TGF-β1 in M group and D group were significantly higher than those in N group, while the D group was significantly lower than that in M group （ P〈0.05）.[Conclu- sion]1, 25 dihydroxycholecalciferol can inhibit expression of TNF-α,TGF-β1 of diabetic rats kidney and take protec- tive effect on renal injury in rats with diabetes.

关 键 词：羟基胆骨化醇类/药理学 糖尿病 2型/并发症 肾/损伤 

分 类 号：R587.1[医药卫生—内分泌]