黄瓜根际土壤细菌群落的16S rDNA-PCR-DGGE分析  

Analysis of the Bacterial Communities in Rhizosphere Soil of Cucumber Using 16S rDNA-PCR- DGGE

作　　者：赵柏霞 闫建芳[2,3] 刘秋 于基成 赵秀香[2] 

机构地区：[1]大连市农业科学研究院,辽宁大连116036 [2]沈阳农业大学植物保护学院,辽宁沈阳110161 [3]大连民族大学生命科学学院,辽宁大连116600

出　　处：《中国蔬菜》2015年第12期33-37,共5页China Vegetables

基　　金：国家自然科学基金项目(31070005;21276047;31270057;31101477);中央高校基本科研业务费自主基金青年项目(DC13010309;DC12010103)

摘　　要：采用PCR-DGGE技术分析了接种枯萎病菌后黄瓜根际土壤细菌群落的动态变化。对黄瓜种植前、接菌前、接菌后发病、接菌后未发病以及接种清水(对照)共5份土壤样品直接提取其总DNA,用F338-GC/R518引物扩增16Sr DNA基因的V3可变区,结合DGGE电泳条带分析样品的细菌群落组成及变化趋势。结果显示,接种枯萎病菌后,会引起黄瓜根际土壤中细菌数量及种类发生较大的变化,其中,接种枯萎病菌后Rhodococcus phenolicus(E1)、Clostridiumsp.(E3、E6)以及3种未培养细菌(E2、E4、E5)数量增加,另外2种未培养细菌(A1、A2)数量减少。16S rDNA-PCR-DGGE was used to analyze the bacterial community dynamic changes in rhizosphere soil infected by cucumber Fusarium wilt. There are 5 rhizosphere soil samples, including pre-planting soil, pre-inoculation soil, diseased cucumber and healthy cucumber after inoculation, not inoculated as the control. Total DNA was directly extracted and amplified with F338-GC and R518 primers targeting the 16S rDNA V3. The amplified were analyzed by DGGE analysis. The result revealed that there was a high diversity of bacterial community compositions among different tested soil samples. Fusarium caused a great bacterial species and number changes in rhizosphere soil, of which Rhodococcus phenolicus, Clostridiurn sp. and 3 uncultured bacteria increased, another 2 uncultured bacteria reduced after inoculation.

关 键 词：根际微生物 PCR-DGGE 枯萎病菌 细菌群落 

分 类 号：S154.3[农业科学—土壤学]