多杀性巴氏杆菌HN06基因组分泌蛋白的预测与分析  

Prediction and Analysis of Secreted Proteins Encoding Genes in Genome of Pasteurella Multocida HN06

作　　者：彭忠[1] 梁婉[1,2] 刘文静[1] 徐卓菲[1] 谭臣[1] 吴斌[1] 周锐[1] 陈焕春[1] 

机构地区：[1]华中农业大学动物医学院农业微生物学国家重点实验室生猪健康养殖协同创新中心,湖北武汉430070 [2]华中农业大学动物科学技术学院农业动物遗传育种与繁殖教育部重点实验室生猪健康养殖协同创新中心,湖北武汉430070

出　　处：《动物医学进展》2015年第12期6-10,共5页Progress In Veterinary Medicine

基　　金：国家科技支撑计划项目(2014BAD20B00);湖北省科技支撑计划项目(2013BBB14)

摘　　要：预测分析多杀性巴氏杆菌HN06株的分泌蛋白并筛选多株巴氏杆菌共有Sec途径分泌蛋白,为研究多杀性巴氏杆菌亚单位疫苗候选蛋白奠定基础。利用Signal P、Tmhmm、Phobius、Target P、Lipop、GPI-SOM、Tat P、SecretomeP对已完成全基因组测序的多杀性巴氏杆菌HN06株进行分泌蛋白预测及功能分析,并结合已公布全基因组序列的多杀性巴氏杆菌Pm70、3480、36950的基因组序列筛选4株多杀性巴氏杆菌中共有的Sec途径分泌蛋白。结果表明,预测出HN06株分泌蛋白共373个,其中Sec分泌蛋白157个,Tat途径分泌蛋白8个,无信号肽的非经典途径分泌蛋白208个。Sec分泌蛋白中被Ⅰ型信号肽酶识别的有120个,被Ⅱ型信号肽酶识别的有37个。再对Pm70、3480、36950株进行Sec途径分泌蛋白的预测,使用blastp筛选它们共有的Sec蛋白114个。经过统计发现,多杀性巴氏杆菌HN06株不同类型分泌蛋白的功能分布各有侧重,其中ABC转运蛋白和铁摄取相关蛋白对于巴氏杆菌毒力因子的研究具有很重要的意义。因此,对分泌蛋白的深入分析对于更清晰地了解巴氏杆菌的致病机制及筛选免疫原性蛋白具有重要意义。To predict and analyze the secretory proteins of Pasteurella multocida HN06 and choose the common Sec proteins among different Pasteurella multocida strains,softwares including Signal P,Tmhmm,Phobius,Target P,Lipop,GPI-SOM,Tat P and SecretomeP were combined to predict the secretory protein of Pasteurella multocida HN06 which was completely sequenced by our laboratory.The shared Sec proteins among Pasteurella multocida Pm70,3480,36950 and HN06were also identified.A total of 373 secreted proteins were identified,including 157Sec-type signal peptides,8Tat-type signal peptides and 208no-classical proteins.Of the total 157 Sec proteins,120 proteins can be recognized by signal peptidase I and 37 proteins by signal peptidase II.Sec proteins of Pm70,3480 and 36950were also predicted,and approximately 114 Sec proteins were shared by them.The predicted data demonstrated that the secretory proteins associated with various functions,and those related to the metabolism,iron uptake may have a significance to study virulence factors of Pasteurella multocida.Our study may shed a light on researching the pathogenic mechanism of the pathogen and even the subunit vaccine candidate proteins against Pasteurella multocida.

关 键 词：多杀性巴氏杆菌 分泌蛋白 预测与分析 

分 类 号：S852.615[农业科学—基础兽医学]