小RNA重组表达质粒转入猪体内环境释放安全性研究概况  

Induction to Environmental Safety of Recombinant Plasmid Expressing microRNA Transforming into Swine

作　　者：寇建平[1] 孙亚妮[2] 赵钦[2] 周恩民[2] 

机构地区：[1]农业部优质农产品开发服务中心,北京100020 [2]西北农林科技大学动物医学院农业部兽用药物与兽医生物技术陕西科学观测实验站,陕西杨凌712100

出　　处：《动物医学进展》2015年第12期161-164,共4页Progress In Veterinary Medicine

基　　金：农业部食品质量安全监管项目(转NMHCⅡ-A人工miRNA猪的安全评价)

摘　　要：人工小RNA(MicroRNA,miRNA)是根据内源miRNA的生成途径人工合成的miRNA,可以模拟内源miRNA的作用方式与靶mRNA碱基互配指导靶mRNA的降解或抑制其翻译,从而对基因表达进行转录后负调控。将人工miRNA转入动物体内调控目的基因表达,已在转基因动物抗病育种方面得到广泛的研究,与此同时其环境释放导致的生物安全性问题也成为国际关注的重要问题。论文综述了以RNA干扰为基础的转人工miRNA抗猪蓝耳病猪在动物逃逸、环境释放以及与转入基因与宿主基因整合等方面对环境生物安全的影响,以期为猪蓝耳病NMHCⅡ-A转基因猪的进一步研究提供生物安全保障。Artificial RNA（microRNA,miRNA）is synthesized based on the expressing process of the endogenous miRNA.The artificial miRNA mocking endogenous miRNA guides degradation of RNA,inhibits RNA translation and negatively regulates the post-transcriptional by binding the target mRNA.The expressions of the target genes can be regulated by transforming artificial miRNA into animals in vivo,which has been widely investigated with regard to transgenic animal disease-resistant breeding.At the same time,the issue of biological safety has become an important problem of international concern.In the paper,the immune escape,environmental release and exogenous gene integration were reviewed in the trans-genetic pigs against PRRSV infection by transforming miRNA into the pig in vivo.It can improve biosecurity for the research on NMHC II-A transgenic pigs infected with PRRSV.

关 键 词：重组表达质粒 基因水平转移 基因组整合 人工miRNA 

分 类 号：S852.2[农业科学—基础兽医学] Q788[农业科学—兽医学]