潍坊及周边地区牛支原体感染状况调查  

Investigation on Mycoplasma bovis Infection Status in Weifang and Surrounding Areas

作　　者：孙霞 胡士林 

机构地区：[1]山东畜牧兽医职业学院,山东潍坊261061

出　　处：《动物医学进展》2015年第12期172-174,共3页Progress In Veterinary Medicine

基　　金：山东省现代农业产业技术体系牛产业创新团队(SDAIT-12-011-05);潍坊市科技发展计划项目(2014GX075)

摘　　要：为了解潍坊及周边地区牛支原体在健康牛场和发生呼吸道病牛场的感染状况,分别采集了健康牛场的188份血清和发病牛场的肺、鼻腔深部分泌物39份,对血清采用间接酶联免疫吸附试验检测其中的牛支原体抗体,对发病牛的肺和鼻腔深部分泌物采用套式PCR检测其中的牛支原体核酸。结果显示,发病牛场的个体阳性率为46.15%(18/39);健康牛场的场群抗体阳性率为64.29%(9/14),健康牛场的个体抗体阳性率平均为5.85%(11/188),其中肉牛的个体抗体阳性率为5.68%(5/88),奶牛的个体抗体阳性率为6%(6/100)。说明牛支原体在受调查地区健康牛场普遍存在,但个体感染率不高,而在发生呼吸道病牛场中的感染率较高。The present study was undertaken to investigate Mycoplasma bovis infection status in bovine farms of Weifang and surrounding areas.Total 188 serum samples of healthy cattle farms and 39 diseased tissue samples were collected.These serum samples were examined using indirect ELISA to determine the presence of Mycoplasma bovis antibodies,and the diseased lung and nasal deep secretions were examined using nested PCR to determine the nucleic acid materials.The result showed that the positive rates in diseased and health cattle were determined to be 64.29%（18/39）and 64.29%（9/14）,and the individual antibody positive rate of healthy cattle averaged 5.85%（11/188）.The individual antibody positive rate of beef cattle and cows were determined to be 5.68%（5/88）and 6%（6/100）.These results indicated the prevalence of Mycoplasma bovis in the area,but the individual infection rate was not high,and the higher infection occurred in the cattle with respiratory disease.

关 键 词：牛支原体 感染情况 间接酶联免疫吸附试验 套式聚合酶链反应 

分 类 号：S852.62[农业科学—基础兽医学]