细菌性阴道病唾液酸酶测定质量控制调查结果分析  

Result analysis on quality control survey of bacterial vaginosis sialidase determination

作　　者：葛平 徐蓉 陈蓉 王敬华 刘学杰 

机构地区：[1]上海市临床检验中心,上海200126

出　　处：《检验医学》2015年第11期1083-1085,共3页Laboratory Medicine

基　　金：上海市卫生和计划生育委员会资助项目(2013ZYJB0010)

摘　　要：目的了解上海地区不同级别医院临床实验室在不同质量调查方式下的细菌性阴道病（BV）唾液酸酶测定结果的质量。方法被调查的临床实验室用唾液酸酶调查品进行BV（唾液酸酶法）的快速检测。对2010～2014年5年15次不同类型的质量调查结果的符合情况分别按不同级别医院实验室（一组以二、三级医院为主，另一组以民营医院为主）进行统计分析。同时分析2012～2014年9种常用的BV快速检测试剂盒不同级别医院实验室的检测结果。结果不同级别医院实验室调查结果的平均符合率相差30．4％；在使用同一品牌试剂盒的情况下，不同级别医院实验室的BV检测结果符合率最大相差50％。结论不同级别医院的临床实验室其BV检测结果差异极大，部分临床实验室的BV检测质量存在严重问题。因此加强临床实验室的检验质量管理和控制是提高BV检测质量的关键。临床实验室在降低检测试剂采购成本的同时必须考虑试剂的质量。日常检测过程中加强室内质量控制也是提高检测质量的关键之一。Objective To investigate the result quality of bacterial vaginosis （BV） sialidase determination in Shanghai hospital clinical laboratories at different levels by a variety of survey methods. Methods The rapid detection of BV （sialidase method） was performed with sialidase investigation among the clinical laboratories. The quality control of the survey results from 2010 to 2014 with 15 different types were analyzed statistically, according to the different levels of hospital laboratories （ a group of Level 2 and 3 hospitals and a group of private hospitals）. From 2012 to 2014, 9 kinds of commonly used BV rapid test kits were used, and the results of different levels of hospital laboratories were analyzed. Results Different levels of hospital laboratory results were consistent with the difference rate of 30.4%. In the case of using same kit, different levels of hospital laboratory results were consistent with BV difference rate of 50%. Conclusions BV results vary widely in different levels of clinical laboratories, and some clinical laboratory quality has serious problems. Strengthening the quality control and management of clinical laboratory determination for BV is important. Clinical laboratories should reduce the costs of kits and ensure the quality. For routine determination, strengthening internal quality control is one of the keys for quality control.

关 键 词：唾液酸酶 细菌性阴道病 质量调查 

分 类 号：Q556[生物学—生物化学]