α-硫辛酸对肥胖模型大鼠血清脂肪因子的影响  

Influence of alpha-lipoic acid on serum adipocytokines in a rat model of dietary-induced obesity

作　　者：邵云[1] 秦小霞[2] 魏从真[2] 张秋芬 邵明 闫国超[2] 

机构地区：[1]河北医科大学第一医院超声科,河北石家庄050031 [2]河北医科大学第一医院检验科,河北石家庄050031 [3]石家庄市桥西区振头社区卫生服务中心,河北石家庄050031

出　　处：《检验医学》2015年第11期1147-1149,共3页Laboratory Medicine

摘　　要：目的探讨α-硫辛酸(ALA)对饮食诱导肥胖大鼠脂肪因子的影响。方法选取清洁级8周龄雄性Wistar大鼠50只,随机选其中10只,以普通饲料喂养作为对照组(NC);高脂饮食成功诱导肥胖大鼠模型32只,随机分为高脂饲料(HFD)组(16只)和HFD+ALA组[16只,腹腔注射ALA(30 mg/kg)2周]。测定各组大鼠血清氧化应激、脂肪因子等指标并进行统计学分析。结果 HFD组血清丙二醛(MDA)、瘦素、肿瘤坏死因子-α(TNF-α)分别为(8.8±0.7)μmol/L、(9.8±0.8)ng/m L、(11.6±1.2)ng/L,明显高于HFD+ALA组[(6.1±0.6)μmol/L、(7.6±0.7)ng/m L、(9.7±2.3)ng/L]和NC组[(7.9±0.4)μmol/L、(4.6±0.6)ng/m L、(8.8±3.5)ng/L](P均<0.05);而超氧化物歧化酶(SOD)[(73.9±5.1)U/m L]、总抗氧化能力(T-AOC)[(18.0±3.6)U/m L]、脂联素[(8.7±1.5)mg/L]明显低于HFD+ALA组[(80.9±1.8)U/m L、(25.8±5.4)U/m L、(12.6±2.1)mg/L]和NC组[(81.0±3.2)U/m L、(20.9±2.3)U/m L、(14.0±3.4)mg/L](P均<0.05)。与NC组比较,HFD+ALA组血清T-AOC、瘦素明显升高(P均<0.05),MDA明显下降(P<0.05),SOD、脂联素、TNF-α差异无统计学意义(P均>0.05)。相关性分析显示血清MDA与瘦素、TNF-α呈明显正相关[相关系数(r)值分别为0.716、0.502,P均<0.01];T-AOC与脂联素呈负相关(r=-0.485,P<0.05)。结论 ALA可改善肥胖大鼠的氧化应激状态并影响脂肪因子的分泌。Objective To investigate the influence of alpha-lipoic acid （ALA） on serum adipocytokines in a rat model of dietary-induced obesity. Methods A total of 50 Wistar rats were randomly classified into 3 groups, a negative control （NC） group fed with normal chow （ 10 cases） , high fat diet（HFD） group fed with a high-fat diet （ 16 cases） and HFD ＋ ALA group fed with a high-fat diet with an intervention of ALA （30mg/kg, 2 weeks, 16 cases ）. The concentrations of serum oxidative stress hiornarkers and adipocytokines were determined, and the results were analyzed statistically. Results The levels of serum malondialdehyde（MDA） , leptin and tumor necrosis factor-alpha （TNF-α） in HFD group were （ 8.8± 0.7 ） txmol/L , （9.8 ± 0. 8 ） ng/mL and （ 11.6 ±1.2 ） ng/L, which were higher than those in HFD＋ALAgroup E（6.1±0.6）μmo]/L , （7.6 ±0.7）ng/mL and （9.7±2.3）ng/L] and NC group [（7.9 ± 0. 4 ） μmol/L, （4.6 ± 0. 6 ） ng/mL and （ 8.8 ± 3.5 ） ng/L ] （ P 〈 0. 05 ）. The levels of superoxide dismutase （ SOD ） （73.9±5.1）U/mL], total antioxidant capacity （T-AOC） [ （ 18. 0 ± 3. 6） U/mE] and adiponectin [ （8. 7 ± 1.5） rag/L] were lower than those in HFD ＋ ALA group [ （80. 9 ± 1.8） U/mL, （25.8 ±5.4） U/mL and（ 12.6 ± 2.1）mg/L] andNC group [（81.0 ±3.2）U/mL,（20.9 ±2. 3）U/mE and （14.0±3.4）rag/El （P 〈0.05）. Compared with NC group, the levels of serum T-AOC and leptin were much higher in HFD ＋ ALA group （ P 〈 0.05 ）, MDA was much lower （ P 〈 0.05 ） , and no statistical significance between the 2 groups was observed for SOD, adiponectin and TNF-α （P 〉 0.05 ）. Moreover, correlation analysis showed that serum MDA , leptin and TNF- α showed a significantly positive correlation [ correlation coefficients （r） = 0. 716 and 0. 502, P 〈 0.01 ]. A negative correlation of T-AOC with adiponectin （ r = - 0. 485,P 〈 0.05 ）. Conclusions ALA can improve the oxidative stress state and

关 键 词：Α-硫辛酸 脂肪因子 肥胖 动物模型 氧化应激 

分 类 号：R446.1[医药卫生—诊断学]