姜黄素对宫颈癌Hela细胞株放疗增敏的机制研究  

Radiotherapy sensitization role of curcumin in Hela cells and its possible mechanisms

作　　者：张莉[1] 邓守恒[1] 陈萍[1] 汤继英[1] 

机构地区：[1]湖北医药学院附属人民医院肿瘤中心,湖北省十堰442000

出　　处：《中国医师杂志》2015年第11期1680-1681,1685,共3页Journal of Chinese Physician

基　　金：湖北省十堰市科技局基金资助项目（ZD2012026）;湖北医药学院第三临床学院资助项目（201201）

摘　　要：目的 探讨姜黄素对宫颈癌Hela细胞放疗增敏作用的可能机制.方法 采用Western-blot法检测姜黄素和放射线单独作用以及放射线与姜黄素联合应用后宫颈癌Hela细胞中p53、p21、Bax、Bcl-2蛋白的表达情况.结果 25μmol/L姜黄素与4Gy的X-射线均能升高p53、p21、Bax蛋白的表达,降低Bcl-2蛋白的表达（P＜0.05）,两者联合作用比单独作用变化更加显著（P＜0.01）.结论 姜黄素对人宫颈癌Hela细胞的放疗增敏作用可能与其上调p53、p21蛋白及下调Bcl2/Bax比例有关.Objective To investigate the mechanisms of radiotherapy sensitization role of curcumin in cervical carcinoma Hela cells.Methods Western blot was used to detect expressions of p53, p21, Bax,and Bcl-2 proteins after cervical cancer Hela cells were treated.Results The expressions of p53 and p21 proteins were increased.The ratio of Bcl-2/Bax was decreased when curcumin and irradiation were applied to cells alone （P 〈 0.05）.This change were much more obvious when irradiation and curcumin were combined than either of them was used alone（P 〈0.01）.Conclusions The mechanisms of curcumin enhancing radiosensitivity on Hela cells might be due to upregulation of p53, p21, and Bax proteins and downregulation of Bcl-2 protein.

关 键 词：姜黄素/药理学 宫颈肿瘤/中药疗法/放射疗法/代谢 肿瘤抑制蛋白质p53/代谢 原癌基因蛋白质p21(ras)/代谢 bcl-2相关X蛋白质/代谢 原癌基因蛋白质c-bcl-2/代谢 

分 类 号：R285[医药卫生—中药学]