饮用水管网生物膜中弧菌的监测  

Survey of Vibrio on biofilm of drinking water distribution system

作　　者：史亚[1] 任红星[2] 罗志逢 柳景青[3] 何晓芳 周晓燕 裘尚德 程东庆[1] 

机构地区：[1]浙江中医药大学医学技术学院卫生检验教研室,浙江杭州310053 [2]浙江大学环境与资源学院环境生态工程研究所 [3]浙江大学建筑工程学院 [4]绍兴市水环境科学研究院有限公司

出　　处：《中国预防医学杂志》2015年第11期831-834,共4页Chinese Preventive Medicine

基　　金：国家自然科学基金项目(51378455);浙江省科技计划项目(2014C33014)

摘　　要：目的调查饮用水管网生物膜中弧菌的种群和数量,为控制弧菌相关疾病提供科学依据。方法采集中国东部某市饮用水管网生物膜样本13份,平皿计数法测定细菌菌落总数,硫代硫酸盐-柠檬酸盐-胆盐-蔗糖（TCBS）琼脂培养基分离培养弧菌,并用弧菌科细菌生化鉴定系统鉴定。结果 13份样本全部检出细菌,检出率为100%;弧菌检出率为53.85%（7/13）;共鉴定海鱼弧菌、霍利斯弧菌和辛辛那提弧菌3种弧菌,检出率分别为30.77%（4/13）、23.08%（3/13）、7.69%（1/13）;弧菌检出数达10^1 CFU/cm^2-2.65×10^3 CFU/cm^2。结论饮用水管网生物膜中存在大量弧菌,对人类健康有潜在的威胁。Objective To investigate the species and amount of Vibrio on biofilm collected from drinking water distribution system,in order to provide the scientific evidence for control of diseases caused by Vibrio. Methods Thirteen biofilm samples were collected from drinking water distribution system.Heterotrophic plate counts（HPC）was used to determine the bacteria amount.The Vibrio bacteria were cultured,isolated by TCBS culture medium,and then identified by biochemical methods. Results The positive rate of bacteria was 100%.Seven samples were positive for Vibrio with the overall positive rate of 53.85%.Vibrio damsel,Vibrio hollisae and Vibrio cincinnatiensis were identified,with the positive rates of 30.77%（4/13）,23.08%（3/13）,7.69%（1/13）,respectively.The amount of Vibrio bacteria ranged from 10^1 CFU/cm^2 to 2.65×10^3CFU/cm^2. Conclusions High amount of Vibrio bacteria exist in biofilm of drinking water distribution system,which were potential risks for public health.

关 键 词：饮用水管网 生物膜 弧菌 

分 类 号：R123.1[医药卫生—环境卫生学]