家鸡G蛋白偶联受体139基因的结构、序列及表达特征分析  

Characterization of the Structure and Tissue Expression of Chicken G Protein-coupled Receptor(GPR139) Gene

作　　者：黄思邈 黄龙[1] 莫春横 李娟[1] 王亚军[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川动物》2015年第6期869-874,共6页Sichuan Journal of Zoology

基　　金：国家自然科学基金项目(31271325;31272436)

摘　　要：G蛋白偶联受体139(GPR139)是一种孤儿受体。在脊椎动物中,针对其结构、表达和功能的研究相对缺乏。本文以家鸡大脑c DNA为模板,采用PCR方法,扩增并克隆得到家鸡GPR139(c GPR139)基因。结果显示:家鸡GPR139基因c DNA由2个外显子组成;ORF区域长1056 bp,编码351个氨基酸的前体蛋白,在其第三跨膜区末端包含D-R-Y基序,属于G蛋白偶联受体家族视紫红质亚家族。将家鸡GPR139前体蛋白与人、大鼠、小鼠GPR139前体蛋白的氨基酸序列进行比对,分别具有91.78%、89.17%、89.17%的相似度。采用RT-PCR方法,本研究也检测了家鸡GPR139的组织表达情况。结果显示:家鸡GPR139基因在大脑、中脑、小脑、后脑、下丘脑及垂体中表达量较高,而在卵巢、精巢、肺、肾脏、肌肉组织中,GPR139基因仅有微弱表达。这些结果首次揭示了GPR139基因在非哺乳动物(家鸡)中的结构特征和组织表达特点,为探究其在家鸡中的生理功能奠定一些基础。G protein-coupled receptor 139（GPR139） is an orphan receptor identified in vertebrates,however,the information regarding its structure,expression and functions remains limited. In this study,the full-length c DNA of chicken GPR139（cGPR139） genes was cloned by PCR. Chicken GPR139 cDNA consisted of two exons,and its open reading frame（ 1056 bp） encoded a protein precursor of 351 amino acids,which shared high amino acid sequence identity with that of human（91.78%）,rat（89.17%） and mouse（89.17%）. The third transmembrane domain of c GPR139 contained a D-R-Y motif,which was typical for rhodopsin subfamily of G protein-coupled receptors. In addition,the expression of GPR139 gene was examined in adult chicken tissues by RT-PCR. The results showed that chicken GPR139 was highly expressed in the pituitary and various brain regions,including telencephalon,midbrain,cerebellum,hindbrain and hypothalamus,but weakly expressed in the ovary,testis,lung,kidney and muscle. The results from present study will pave the way for further investigation of the roles of GPR139 in chickens.

关 键 词：家鸡 GPR139 G蛋白偶联受体 中枢神经系统 

分 类 号：Q78[生物学—分子生物学]