高糖对小鼠骨髓间充质干细胞增殖的影响  

The effect of high glucose on the proliferation of bone marrow-derived mesenchymal stem cells of mice

作　　者：韦恩秀[1,2] 吴冲云 朱玲[1] 李方晖[3] 李星儿[1] 史新平[1] 刘承宜[1] 

机构地区：[1]华南师范大学体育科学学院激光运动医学实验室,广东广州510006 [2]广西师范大学体育学院,广西桂林541004 [3]肇庆学院体育与健康学院,广东肇庆526061

出　　处：《体育学刊》2015年第6期139-144,共6页Journal of Physical Education

基　　金：国家自然科学基金项目(60878061);高等学校博士学科点专项科研基金(博导类)(20124407110013);广东省科技计划项目(社会发展)(2012B031600004;2014A020220015);广东省高等学校青年创新人才项目(2014KQNCX225)

摘　　要：采用不同浓度葡萄糖对间充质干细胞（MSCs）增殖的影响来研究运动员补糖的MSCs效应,分别用5.0 和22.5 mmol/L 葡萄糖预处理小鼠MSCs 细胞株C3H10T1/2 48 h 后,换不同浓度葡萄糖（5.0-500.0 mmol/L）持续培养或50.0-150.0 mmol/L 和22.5 mmol/L 进行12 h 交替培养,甲基噻唑基四唑（MTT）法检测24-192 h 的细胞增殖活性.结果发现：1）22.5 和50.0 mmol/L 葡萄糖组细胞的增殖活性最佳,并称为正糖.浓度低于或高于正糖的葡萄糖称为低糖或高糖.2）22.5 mmol/L 正糖预处理后换不同糖浓度持续培养时,和正糖相比,高糖100.0-300.0 mmol/L 具有短暂促增殖作用.3）高糖长期培养抑制细胞增殖.结果说明：高糖对正糖预处理的MSCs 具有短暂促增殖效应.In order to study the mesenchymal stem cells (MSCs) effect of carbohydrate supplementation for athletes by studying the effect of glucose in different concentrations on the proliferation of MSCs, the authors pretreated cell lines C3H10T1/2 of MSCs of mice with 5.0 and 22.5 mmol/L glucose respectively for 48 h, then continuously cul-tured them with glucose in different concentrations (5.0~500.0 mmol/L) or alternatively cultured them with 50.0~150.0 mmol/L and 22.5 mmol/L glucose for 12 h, measured the proliferation activity of 24~192 h cells by means of methylthiazolyldiphenyl-tetrazolium bromide/thiazolyl blue tetrazolium bromide (MTT) assay, and re-vealed the following findings: 1)the proliferation activity of the cells cultured with 22.5 and 50.0 mmol/L glucose was the best, and the glucose was called as normal glucose, while the glucose whose concentration was lower or higher than normal glucose was called as low glucose or high glucose; 2)when MSCs were pretreated with 22.5 mmol/L normal glucose and then continuously cultured with glucose in different concentrations, as compared with normal glucose, 100.0~300.0 mmol/L high glucose had a short-term proliferation promoting function; 3)longer-term high glucose culturing restrained cell proliferation. The said findings indicate that high glucose has a short-term pro-liferation promoting effect on MSCs pretreated with normal glucose.

关 键 词：运动生物化学 间充质干细胞 正糖 高糖 小鼠 

分 类 号：G804.7[文化科学—运动人体科学]