表面有机改性纯镁的体外生物相容性分析  

Hemolysis and cytotoxicity of surface organically modified pure magnesium

作　　者：乔丽英[1,2] 余重阳 龚才华 谢明辉[1] 王维朗[1] 王勇[1,2] 

机构地区：[1]重庆大学材料科学与工程学院,重庆400045 [2]重庆大学国家镁合金工程技术研究中心,重庆400044

出　　处：《功能材料》2015年第24期24048-24052,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(51101174);重庆市自然科学基金资助项目(cstc2011jj A50008);国家支撑计划资助项目(2012BAI18B02);重庆市应用开发计划资助项目(cstc2014yykf C50002)

摘　　要：在纯镁表面制备植酸和硅烷有机改性膜层,考察改性镁的溶血性和细胞毒性。实验结果表明,改性材料的溶血率出现明显下降,纯镁表现为57.39%,而植酸改性镁表现为4.28%,硅烷改性镁表现为0.87%,经过改性的镁的溶血率符合生物材料的要求。植酸改性镁浸提液对成骨细胞的毒性在1 d后表现为2级有毒,3和5 d后分别为2级无毒和1级无毒;1 d的毒性不能满足生物材料的要求。硅烷改性镁浸提液对成骨细胞的毒性在1和3 d后表现均为1级无毒,而且在5 d后为0级,表现出对细胞生长的促进作用。Phytic acid and silane were selected to modify the pure magnesium in this study. Hemolysis and cytotoxicity of modified magnesium were tested. The results show that compared with pure magnesium,the hemolysis rate of modified materials dropped significantly. The hemolysis rate of pure magnesium was 59. 93%,while the magnesium modified by phytic acid was 4. 28 and by silane was 0. 87%,which are within the demand( < 5%). The cytotoxicity of phytic acid modified magnesium was level 2 toxic after 1 d,cannot meet the requirements of biomaterials; then change to level 2 and level 1 non-toxic after 3 and 5 d respectively,which meet the requirements of biomaterials. The cytotoxicity of silane modified magnesium meets the demand of cytoxicity through the whole test,was level 1 of non-toxic after 1 and 3 d,while after 5 d was level 0 and has a promoting effect on the growth of cells.

关 键 词：生物相容性 细胞毒性 溶血率 纯镁 植酸 硅烷 

分 类 号：TG178[金属学及工艺—金属表面处理] R318.08[金属学及工艺—金属学]