昙花中槲皮素、异鼠李素和山奈酚量的测定  

Simultaneous determination of quercetin,kaempferol and isorhamnetin in Epiphyllum oxypetalum by HPLC

作　　者：邹翔[1] 盛洁静[2] 曲中原[2] 张振国[3] 徐阳[3] 季宇彬[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,哈尔滨150076 [2]哈尔滨商业大学药学院,哈尔滨150076 [3]哈尔滨商业大学英才学院,哈尔滨150028

出　　处：《哈尔滨商业大学学报（自然科学版）》2015年第5期513-515,526,共4页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金青年基金项目(81102858);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015070);黑龙江省博士后资助项目(LBH-Z11102);哈尔滨商业大学大学生创新创业训练计划国家级项目(201410240007)

摘　　要：建立高效液相法同时测定昙花中含槲皮素、山奈酚和异鼠李素的量.运用Diamonsil C18色谱柱为分析柱,以甲醇-0.4%磷酸水溶液（55∶45）为流动相,流速为1.0 m L/min,检测波长为360 nm.测得槲皮素在5.5~88μg/m L范围内呈良好线性关系,r=0.999 9（n=6）;山奈酚在4~64μg/m L范围内呈良好线性关系,r=0.999 9（n=6）;异鼠李素在6.5~104μg/m L范围内呈良好线性关系,r=0.999 9（n=6）,三者的回收率分别为（99.84±0.23）%（RSD=0.24%）、（100.20±0.52）%（RSD=0.38%）、（99.99±0.12）%（RSD=0.11%）.槲皮素、异鼠李素和山奈酚的量分别为0.05、0.026、0.039 mg/g.本方法可同时测定昙花中槲皮素、异鼠李素和山奈酚,操作简单、灵敏、重复性好,适用于昙花及其制剂的质量控制.A rapid and sensitive HPLC method was developed to detect the content of querce-tin, kaempferol and isorhamnetin in Epiphyllum oxypetalum .Diamond -C18 column was used as chromatographic column , methanol-0.4%phosphoric acid （55∶45） water solution as mobile phase , and the detection wavelength was set at 360 nm, with a flow rate at 1.0 mL/min.There was a good linear relationship when quercetin was in the range of 5.5~88μg/mL, r=0.999 9（n=6）, kaempferol was in the range of 4~64 μg/mL, r=0.999 9（n=6） and isorhamnetin was in the range of 6.5~104 μg/mL, r=0.999 9（n=6）.The av-erage recoveries were （99.84 ±0.23）% （ RSD=0.24%）, （100.20 ±0.52）% （ RSD=0.38%）, 99.99 ±0.12% （RSD=0.11%） respectively.The result showed that the con-tent of quercetin, kaempferol and isorhamnetin in Epiphyllum oxypetalum were 0.05, 0. 026, 0.039 mg/g, respectively.The established method was simple , sensitive and repro-ducible to detect the content of quercetin , kaempferol and isorhamnetin in Epiphyllum oxyp-etalum at the same time , which could be applied in the quality control of Epiphyllum oxyp-etalum and its preparations .

关 键 词：昙花 槲皮素 山奈酚 异鼠李素 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]