HPLC法测定槲寄生中N-桂皮酰基丁二胺  

Determination of N-cinnamoyl putrescine in Viscum coloratum( Kom. ) Nakai by HPLC

作　　者：孙永慧[1] 郎悦[1] 张颖[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2015年第5期530-532,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省教育厅科学技术研究项目(12531635)

摘　　要：建立HPLC法测定槲寄生中N-桂皮酰基丁二胺的量的方法.采用高效液相色谱法检测,DiamonsilTMC18色谱柱（5.6×250 mm,5μm）;以乙腈-0.2%磷酸溶液（20∶80）为流动相;检测波长：278nm;流速：1.0m L/min;柱温：25℃.N-桂皮酰基丁二胺在0.1~0.5μg（r=0.999 8）范围内呈良好的线性关系;平均回收率为98.3%（n=6）,RSD=0.8%.该测定方法准确、简便、灵敏度高、重现性好,可作为该药材的质量控制方法.An assay method was established for the determination of N -cinnamoyl putrescine in Viscum coloratum （Kom.） Nakai by HPLC.DiamonsilTM C18 analytical column （250 mm ×5.6 mm, 5 μm） was used with a mobile phase of aceronitrile -0.2% H3PO4（20∶80）. The flow rate was 1.0 mL/min.The detecting wavelength was at 278 nm.The column tem-perature was at 25℃.The linear range of N-cinnamoyl putrescine was 0.1~0.5 μg （ r=0.999 8）.The average recovery was 99.9%.RSD=1.80%.The method was accurate, simple , highly sensitive and reproducible .It can be used for the quality control of Visum coloratum （ Kom.） Nakai.

关 键 词：槲寄生 N-桂皮酰基丁二胺 高效液相色谱 

分 类 号：R284[医药卫生—中药学]