基于UPLC/Q-TOF MS代谢组学技术研究远志不同品种间的化学差异性  

UPLC/Q-TOF MS-Based Metabolomics Analysis of Chemical Differences in Different Polygala tenuifolia Varieties

作　　者：白璐[1,2] 许晓双 张福生[1] 彭冰[3] 曾祖平[3] 田洪岭[4] 秦雪梅[1] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学化学化工学院,山西太原030006 [3]北京市中医研究所/首都医科大学附属北京中医医院,北京100010 [4]山西省农业科学院经济作物研究所,山西汾阳032200

出　　处：《中药材》2015年第9期1819-1824,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31100244);山西省科学技术发展计划(20140313010-1);国家科技支撑计划(2011BA107B05);山西省创新团队(2013131015)

摘　　要：目的:通过对远志不同品种-晋远1号(简称JY1)、汾远2号(简称FY2)及传统汾阳产远志(简称FY)的差异性研究,以期为筛选远志良种提供参考。方法:采用超高效液相色谱-四极杆-飞行时间质谱(UPLC/Q-TOF MS)对JY1、FY2、FY样品进行测定,采用主成分分析(PCA)等多种统计分析方法,进行代谢组学分析并找出差异代谢物。结果:与FY相比,JY1及FY2中的蔗糖酯(如sibiricoses A5、tenuifoliside B等)及低聚糖(如tenuifoliose K等)对PCA分类贡献较大;而与JY1相比,FY2中的蔗糖酯(如tenuifoliside B、tenuifoliside A等)及低聚糖(如tenuifoliose A等)对PCA分类也有较大贡献。此外,sibiricaxanthone A、3,6'-disinapoly sucrose及seneginⅢ在FY、JY1、FY2中的相对含量也有较大差异。结论:与FY相比,JY1与FY2作为远志的新品种,在化学物质基础上存在一定差异且各具优势,这为今后远志的良种定向选育(即以体内某种或某几种活性物质含量高低为依据)提供了数据支持。Objective： The chemical differences of Polygala tenuifolia varieties-Jin Yuan 1（ JY1）,Fen Yuan 2（ FY2） and traditional Fen Yang（ FY） were studied,in order to provide reference for the breeding of Polygala tenuifolia. Methods： The samples of JY1,FY2 and FY were subjected to ultra-high performance liquid chromatography（ UPLC） quadrupole time-of-flight mass spectrometry（ Q-TOF MS） analysis. The obtained data were analyzed using Principal Component Analysis（ PCA） and other statistical analysis methods,and differential metabolites were further figured out. Results： Compared with FY,sucrose esters（ such as sibiricoses A5 and tenuifoliside B） and oligosaccharides（ such as tenuifoliose K） in JY1 and FY2 contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot. Compared with JY1,The sugar esters（ such as tenuifoliside B and tenuifoliside A） and oligosaccharides（ such as tenuifoliose A） in the FY2 also contributed more to the separation of Polygala tenuifolia varieties in the PCA score plot. In addition,the relative contents of sibiricaxanthone A,3,6＇-disinapoly sucrose and senegin Ⅲ showed significant differences among FY,JY1 and FY2. Conclusion：As new Polygala tenuifolia varieties,JY1 and FY2 had certain differences and respective advantages on the chemical composition compared with FY,which could provide data support for the directional breeding of Polygala tenuifolia based on the contents of some active compounds.

关 键 词：远志 代谢组学 晋远1号 汾远2号 UPLC/Q-TOF MS 

分 类 号：R284.1[医药卫生—中药学]