血液灌流对灭多威中毒兔心肌的保护作用及其机制  

Protective Effect and its Mechanism of Hemoperfusion on Methomyl Poisoning Rabbit Myocardium

作　　者：杨婷[1] 曹云刚[2] 王征[1] 王志翊[1] 

机构地区：[1]温州医科大学附属第二医院急诊科,浙江温州325000 [2]温州医科大学附属第二医院神经内科,浙江温州325000

出　　处：《中国药师》2015年第12期2070-2072,共3页China Pharmacist

基　　金：浙江省自然科学基金(编号:LY15H150008);温州市科技计划项目(编号:Y20140493)

摘　　要：目的:观察血液灌流(HP)对灭多威中毒兔损伤心肌的保护作用及其机制。方法:24只兔随机分为3组:正常对照组(N组)、中毒组(P组)、血液灌流组(HP组),每组8只,P组灭多威中毒模型,假血液灌流2h,HP组兔灭多威中毒模型,行血液灌流2 h,观察光镜下心肌组织的病理学改变特点,采用比色法检测各组兔心肌组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,应用SPSS16.0软件对实验所得数据进行统计。结果:光学显微镜观察下,P组心肌组织可见充血、水肿等病理学改变,HP组与N组心肌组织充血水肿等病理改变不明显;与N组,HP组比较,P组实验兔心肌组织SOD活性明显下降,差异有统计学意义(P<0.05),P组MDA含量有明显升高差异具有统计学意义(P<0.05)。结论:早期血液灌流可减轻灭多威中毒所致心肌组织损伤,其机制与血液灌流的抗氧化损伤有关。Objective： To observe the effect and its mechanism of hemoperfusion on injured myocardium under acute toxication of methomyl in rabbits. Methods： Twenty-four rabbits were randomly divided into three groups： control group （N group）, acute metho- myl intoxication group （P group）, hemoperfusion group （HP group）. P group and HP group was treated by methomyl intoxication, and with 2-hour sham-hemoperfusion and hempoperfusion respectively. Then the pathological changes of myocardium were observed under optical microscope. The activity of SOD and content of MDA in myocardium were measured by calorimetry. The experimental data were analyzed by SPSS 16.0 package. Results： There were congestion, edema and other pathological changes in group P, while no obvious changes in N group and HP group. The activity of SOD decreased while MDA content increased in P group versus N and HP group （ P 〈 0.05 ）. Conclusion： Hemoperfusion can alleviate the myocardial injury induced by methomyl poisoning through the mechanism of the resistance to oxidative damage.

关 键 词：血液灌流 灭多威 心肌 超氧化物歧化酶 丙二醛 

分 类 号：R965[医药卫生—药理学]