FTY720对实验性肝纤维化小鼠肝损伤的保护作用  

Protective effect of FTY720 on hepatic injury in experimental hepatic fibrosis mice

作　　者：张宸豪[1] 李瑶[1] 陈为[1] 赵良中[1] 李妍[1] 

机构地区：[1]吉林医药学院检验学院病原生物学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2015年第6期1154-1157,I0013,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(82102953);吉林省科技厅中青年科技创新领军人才及团队资助课题(20130521018JH);吉林省科技厅重点科技攻关项目资助课题(20140203012YY);吉林省教育厅"十二五"科学技术研究项目资助课题(2012第487号);吉林省卫生厅重点专科项目资助课题(2013Z091)

摘　　要：目的:观察FTY720对刀豆蛋白A(Con A)所致肝纤维化小鼠的保护作用,探讨其可能的作用机制。方法:采用刀豆蛋白A(Con A)建立小鼠肝纤维化动物模型,取40只BALB/C小鼠随机分为空白对照组、模型组、FTY720低剂量(1mg·kg-1)组和FTY720高剂量(4mg·kg-1)组,每组10只。测定各组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性和肝脏指数,观察肝脏组织学病理变化。结果:FTY720低剂量组和高剂量组ALT、AST活性明显低于模型组(P<0.05或P<0.01)。光学显微镜观察,模型组有炎性细胞浸润,肝细胞坏死。Masson染色,模型组血管周围有明显纤维条束,肝小叶发生融合;与模型组比较,FTY720低剂量组和高剂量组损伤程度较轻,且随FTY720剂量增加肝组织损伤程度逐渐减轻。结论:FTY720对实验性肝纤维化小鼠的保护作用可能是通过降低ALT和AST活性,进而减轻肝脏损伤程度而延缓纤维化进程。Objective To observe the protective effects of FTY720 on the Con A-induced mouse hepatic fibrosis injury,and to find the possible mechanisms of protective effects.Methods The pathologic models of hepatic fibrosis injury in the mice caused by Con A were set up.Forty mice were randomly divided into control group, model group,high dose of FTY720 （4 mg·kg-1 ）group and low dose of FTY720 （1 mg·kg-1 ）dose group （n=10）.The serum alanine aminotransferase （ALT）and asparate aminotransferase （AST）activities,hepatic index and pathological changes of hepatic tissue were detected .Results Compared with model group,the serum ALT and AST activities in low and high doses of FTY720 groups were decreased significantly （P 〈 0.05 or P 〈 0.01）.The optical microscope results showed that there were inflammatory cells and hepatocellular necrosis in model group. The masson staining results showed that there were surrounding fiber bundle and hepatic lobule fusion in model group;compared with model group,the damage degree in low and high doses of FTY720 groups was reduced.The protective effects of FTY720 on hepatic injury showed linear relation to the drug dose.Conclusion FTY720 could decrease the levels of ALT/AST,thus FTY720 alleviate hepatic damage degree and delay the process of hepatic fibrosis.The protective effects of FTY720 on hepatic injury in experimental hepatic fibrosis mice may be related to the mechanisms mentioned above.

关 键 词：FTY720 刀豆蛋白A 肝纤维化 肝损伤 动物模型 

分 类 号：R392.12[医药卫生—免疫学] R332[医药卫生—基础医学]