原发免疫性血小板减少症患者外周血Th17和Th22及其细胞因子关系研究  

Relationship between Th17 and Th22 in patients with primary immune thrombocytopenia

作　　者：唐海平[1] 谭建国[1] 

机构地区：[1]重庆三峡中心医院输血科,重庆404100

出　　处：《海南医学院学报》2016年第2期144-146,150,共4页Journal of Hainan Medical University

基　　金：重庆市万州区科委科技计划项目(2005407)~~

摘　　要：目的:探讨外周血Th17及其分泌的细胞因子白细胞介素-17(IL-17)和Th22及其分泌的细胞因子IL-22、IL-6、肿瘤坏死细胞因子α(TNF-α)与原发性免疫血小板减少症(ITP)的发病及预后的关系,分析糖皮质激素对Th17和Th22及其细胞因子水平的影响。方法:应用流式细胞仪测定52例ITP患者及50例正常体检者外周血Th17及Th22细胞,ELISA法测定ITP患者及正常体检者IL-17、IL-22、IL-6及TNF-α表达水平。分析ITP患者Th17和Th22及其细胞因子表达水平与预后的关系。结果:ITP组外周血Th17比例、Th22比例及IL-17、IL-22、IL-6、TNF-α水平高于对照组(P<0.05)。糖皮质激素组外周血Th17比例、Th22比例及IL-17、IL-22、IL-6、TNF-α水平显著低于非糖皮质激素治疗组(P<0.05)。完全有效(CR)组及有效(PR)组外周血Th17比例、Th22比例及IL-17、IL-22、IL-6、TNF-α水平显著低于无效组(NR),差异有统计学意义(P<0.05)。结论:Th17及Th22可能参与ITP的发生及病情进展过程中,且其细胞因子可作为患者病情预后评价指标。糖皮质激素可通过抑制外周血Th17比例、Th22比例而起到治疗作用。Objective： To investigate the relationship between Thl7, cytokine interleukin-17 （IL-17）, Th22 cytokines, IL-22, IL 6, tumor necrosis cytokines a （TNF-α） and primary immune thrombocytopenia （ITP）. Methods： Thl7 and Th22 cells of 52 ITP patients and 50 normal volunteers were measured by flow cytometry. The levels of IL-17, IL-22, IL 6 and TNF-α of two groups were measured by ELISA. The relationship of Th17,Th22 and their cytokine with ITP were analyzed. Results.. The levels of Th17, Th22 ratio and IL-17, IL 22, IL-6, TNF-α of ITP group were higher than control groups（t= 3. 986,3. 278, 6.235, 10.784, 4. 526, 5. 612,P〈0. 05）. The levels of Th17, Th22 ratio and IL-17, IL 22, IL-6, TNF-a of glucocorticoids proportion group were lower than non-glucocorticoid therapy group （F= 5. 023, 3. 148, 6. 178, 7. 011, 6. 052, 4. 285,P〈0.05）. The levels of Th17, Th22 ratio and IL-17, IL-22, IL-6, TNF-α of complete remission （CR） group and par-tial response （PR） were significantly lower than the no-remission group （NR）, the difference was statistically significant （F= 5. 789, 6. 023, 5. 986, 6. 785, 6. 302, 6. 782,P〈0.05）. Oonclusion： Thl？ and Th22 may participate in the occurrence and progression of ITP. Its cytokines can be used as indicators in prognosis. Glucocorticoids can inhibit peripheral Th17, Th22,then play a therapeutic role.

关 键 词：原发免疫性血小板减少症 TH17 Th22 细胞因子 

分 类 号：R558.2[医药卫生—血液循环系统疾病]