柴朴汤含药血清对支气管上皮细胞TLR-4及NF-κB表达的影响  

Effects of Chaipu decoction serum on the expressions of TLR-4 and NF-κB on bronchial epithelial cells

作　　者：刘鑫[1] 刘穆华 罗晓青[1] 熊花[3] 

机构地区：[1]南华大学附属第一医院中医科,湖南衡阳421001 [2]南华大学,湖南衡阳421001 [3]益阳市中心医院呼吸科,湖南益阳413000

出　　处：《现代医学》2015年第8期951-956,共6页Modern Medical Journal

基　　金：湖南省中医药管理局课题(201249)

摘　　要：目的:观察柴朴汤含药血清对支气管上皮细胞(bronchial epithelial cells,HBECs)中核转录因子-κB(nuclear transcription factor-kappa B,NF-κB)及Toll样受体-4(Toll-like receptor 4,TLR-4)表达的影响,探讨柴朴汤治疗哮喘的机制。方法:制备柴朴汤含药血清及哮喘血清。将体外培养的气道上皮细胞分为6组进行处理:正常血清组(A组),柴朴汤高剂量血清+哮喘血清组(B组),柴朴汤中剂量血清+哮喘血清组(C组),柴朴汤低剂量血清+哮喘血清组(D组),生理盐水+哮喘血清组(E组),哮喘血清组(F组),采用免疫细胞化学及real-time PCR检测哮喘血清及不同剂量的柴朴汤含药血清对支气管上皮细胞中TLR-4、NF-κB蛋白及mRNA表达的影响。结果:TLR-4、NF-κB蛋白及mRNA在A组表达最弱,经哮喘血清干预后E、F组TLR-4、NF-κB蛋白及mRNA表达均明显增强,与A组比较差异有统计学意义(P<0.05);柴朴汤干预后B、C、D组NF-κB蛋白及mRNA表达下降,与E、F组比较差异有统计学意义(P<0.05)。结论:哮喘血清可诱导气道上皮细胞TLR-4及NF-κB表达明显增强,而柴朴汤含药血清能下调哮喘血清诱导的支气管上皮细胞中TLR-4及NF-κB的表达。Objective： To observed the Chaipu decoction serum＇s affection for the expressions of Toll-like receptors 4（ TLR-4） and nuclear transcription factor-kappa B（ NF-κB） in the airway epithelial cells,to investigete the mechanism of Chaipu decoction treatment of asthma. Methods： The Chaipu decoction and asthma serum were preparerd. The in vitro cultured HBECs were randomly divided into six groups： normal serum group（ Group A）,Chaipu decoction high dose ＋ asthma serum group（ Group B）,medium Chaipu dose ＋ asthma serum group（ Group C）,medium Chaipu dose ＋ asthma serum group（ Group D）,saline serum ＋ asthma serum group（ Group E）,asthma serum group（ Group F）. The expressions of the mRNA and protein of NF-κB and TLR-4 in the different groups were respectively detected by RT-PCR and immunohistochemical method. Results： The expression levels of TLR-4,NF-κB mRNA and protein in Group A were in the minimum amount,with asthmatic serum intervention the expression levels of TLR-4,NF-κB mRNA and protein in Group E,F were significantly higher than those in Group A（ P〈0. 05）. The expression levels of TLR-4,NF-κB mRNA and protein in Group B,C,D（ with Chaipu decoction serum intervention） were significantly lower than those in Group E,F（ P〈0. 05）. Conclusion：Asthmatic secrum can increase the expression of NF-κB and TLR-4 in airway epithelial cells. Chaipu decoction serum can regulate the expressions of TLR-4,NF-κB in airway epithelial cells.

关 键 词：哮喘 气道上皮细胞 柴朴汤含药血清 核转录因子-κB TOLL样受体-4 

分 类 号：R562.25[医药卫生—呼吸系统]