HPLC-DAD法测定不同产地欧亚旋覆花中7种活性成分  

Determination of seven active constituents in Inula britannica from various habitats by HPLC-DAD

作　　者：郭晓民[1] 瞿晶田[1] 柴士伟[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193

出　　处：《现代药物与临床》2015年第11期1328-1331,共4页Drugs & Clinic

摘　　要：目的：建立HPLC-DAD法同时测定欧亚旋覆花中绿原酸、1,5-二咖啡酰奎宁酸、芦丁、菠叶素、槲皮素、木犀草素、6-甲氧基木犀草素。方法 Diamonsil C18色谱柱（200 mm×4.6 mm，5μm）；流动相为乙腈–0.4%枸橼酸水溶液，梯度洗脱；体积流量1 mL/min；柱温30℃；检测波长360 nm；进样量：10μL。结果绿原酸、1,5-二咖啡酰奎宁酸、芦丁、菠叶素、槲皮素、木犀草素、6-甲氧基木犀草素分别在0.118～2.360、0.261～5.220、0.030～0.600、0.052～0.140、0.081～0.162、0.041～0.820、0.030～0.600μg与峰面积呈良好的线性关系。平均回收率分别为97.22%、97.43%、97.52%、99.48%、98.87%、98.76%、98.39%，RSD 值分别为0.92%、1.16%、1.50%、1.56%、0.79%、0.76%、1.62%（n＝6）。结论该方法准确、简便、重复性好，可用于欧亚旋覆花药材中成分的质量控制。Objective To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, 1,5-dicaffeoylquinic acid, rutin, spinacetin, quercetin, luteolin, and 6-methoxyluteolin inInula britannica from various habitats.Methods The analysis was performed on Diamonsil C18 column （250 mm × 4.6 mm, 5μm） with 0.4% citric acid-acetonitrile as mobile phases for gradient elution. The flow rate was 1.0 mL/min. The detection wavelength was set at 360 nm. The column temperature was 30℃ with injection volume of 10μL.Results The calibration curves were linear in the ranges of 0.118 — 2.360, 0.261 — 5.220, 0.030 — 0.600, 0.052 — 0.140, 0.081 — 0.162, 0.041 — 0.820, and 0.030 — 0.600μg for chlorogenic acid, 1,5-dicaffeoylquinic acid, rutin, spinacetin, quercetin, luteolin, and 6-methoxyluteolin, respectively. The average recoveries were 97.22%, 97.43%, 97.52%, 99.48%, 98.87%, 98.76%, and 98.39% with RSD values of 0. 92%, 1.16%, 1.50%, 1.56%, 0.79%, 0.76%, and 1.62% （n= 6）, respectively.Conclusion The method is accurate, convenient and reproducible for quality control for multicomponent inI. britannica.

关 键 词：欧亚旋覆花:绿原酸 l 5-二咖啡酰奎宁酸 芦丁 菠叶素 槲皮素 木犀草素 6-甲氧基木犀草素 HPLC-DAD 

分 类 号：R286.02[医药卫生—中药学]