玉米CDK抑制子ICK基因家族的生物信息学分析  

Bioinformatics analysis of the CDK inhibitor ICK gene family in maize

作　　者：高清松[1] 杜灿灿 杨泽峰[2] 徐辰武[2] 

机构地区：[1]淮阴师范学院江苏省环洪泽湖生态农业生物技术重点实验室/江苏省区域现代农业与环境保护协同创新中心,江苏淮安223300 [2]扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2015年第3期47-53,78,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展计划项目(973-2011CB100106);国家自然科学基金资助项目(31371632)

摘　　要：采用生物信息学方法对玉米基因组中的细胞周期蛋白依赖的激酶（CDK）抑制子（ICK）基因进行了全面鉴定,并对其基因结构、蛋白质保守序列和基因表达情况进行了分析。结果表明：玉米基因组中含有9个ICK基因,片段复制是玉米ICK基因家族数目扩增的主要动力。玉米ICK基因的外显子数为2-4个不等,同一亚族基因的外显子/内含子结构具有一定保守性。蛋白质保守序列分析鉴定出6个保守的基序,这些基序与ICK蛋白的核定位、功能和稳定性调节以及对CDK活性的抑制相关,说明玉米ICK蛋白可能是有功能的CDK抑制子。基于基因芯片和EST数据的表达模式分析表明,多数玉米ICK基因在各组织器官中均有表达,部分基因的表达具有一定的组织特异性。The cyclin-dependent kinase（CDK）inhibitor（ICK）genes in the maize genome were comprehensively identified,and their gene structure,protein conserved sequences and expression profiles were explored.The maize genome harbored nine ICK genes;segmental duplication was the main force underlying the expansion of this family.The exon numbers varied from two to four among maize ICKgenes and the exon/intron structures were similar within each subfamily.Protein conserved sequence analysis identified six motifs,which were known to be involved in the nuclear localization,function and stability regulation and CDK activity inhibition of ICK proteins,suggesting that maize ICK proteins were active CDK inhibitors.Expression profile analysis using microarray and EST data revealed that most maize ICKgenes were expressed in diverse tissues and/or organs;some genes exhibited tissue-preferential expression.

关 键 词：玉米 ICK基因 基因家族 生物信息学分析 

分 类 号：S513[农业科学—作物学]