FPIA法和HPLC-MS/MS法测定甲氨蝶呤血药浓度的相关性研究  

Correlation between FPIA and HPLC-MS/MS in the determination of methotrexate in human plasma

作　　者：朱乐亭[1] 杨莉[1] 赵志刚[1] 赵明[2] 梅升辉[1] 

机构地区：[1]首都医科大学附属北京天坛医院药学部,北京100050 [2]首都医科大学化学生物学与药学院,北京100069

出　　处：《中南药学》2015年第11期1146-1150,共5页Central South Pharmacy

基　　金：国家自然科学基金(No.81301118);多肽及小分子药物北京市重点实验室开放课题(No.2014DTYW03);首都医科大学本科生科研创新项目(No.XSKY2015181)

摘　　要：目的评价荧光偏振免疫分析法（FPIA）和液质联用法（HPLC-MS/MS）测定人血浆中甲氨蝶呤（MTX）浓度的相关性。方法分别用FPIA法和HPLC-MS/MS法测定人血浆中MTX的浓度,并比较两者的相关性。结果当MTX浓度在0.05-1μmol·L^-1时,Wilcoxon检验结果表明FPIA法和HPLC-MS/MS法的测定结果差异有统计学意义,回归方程为：CFPIA=1.0923·CHPLC-MS/MS＋0.001 084,r=0.975。当MTX浓度在1-100μmol·L^-1时,配对t检验结果表明FPIA法和HPLC-MS/MS法的测定结果差异无统计学意义,回归方程为：CFPIA=0.9811·CHPLC-MS/MS-0.4405,r=0.939。结论 FPIA法和HPLC-MS/MS法是否存在显著性差异取决于MTX浓度,MTX浓度可通过公式换算后供临床医师参考。Objective To compare the correlation between fluorescence polarization immunoassay（FPIA） and HPLC-MS/MS methods in the determination of methotrexate（MTX） concentration in human plasma. MethodsThe plasma concentrations of MTX was assayed by FPIA and HPLC-MS/MS respectively. The results determined by both methods were evaluated. Results Significant difference between FPIA and HPLC-MS/MS was showed by Wilcoxon test when MTX concentration was at 0.05- 1 μmol · L^- 1. The regression equation was： CFPIA=1.0923 · CHPLC-MS/MS＋ 0.001 084, r = 0.975. Insignificant difference between FPIA and HPLC-MS/MS was found by paired sample t-test when MTX concentration was at 1- 100 μmol · L^- 1. The regression equation was： CFPIA= 0.9811 · CHPLC-MS/MS- 0.4405, r = 0.939. Conclusion Correlation between FPIA and HPLC-MS/MS depends on the MTX concentration.

关 键 词：荧光偏振免疫分析法 液质联用法 甲氨蝶呤 相关性 治疗药物监测 

分 类 号：R969.1[医药卫生—药理学]