HPLC-DAD法同时测定小儿清解颗粒中9种成分的含量  

Simultaneous determination of 9 components in Xiaoer Qingjie granules by HPLC-DAD

作　　者：李云[1] 刘荣荣[1] 刘思燚 张秀平[1] 刘颖[2] 张加余[2] 卢建秋[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学科研试验中心,北京100029

出　　处：《中南药学》2015年第11期1195-1198,共4页Central South Pharmacy

摘　　要：目的建立HPLC-DAD法同时测定小儿清解颗粒中9种成分（新绿原酸、绿原酸、隐绿原酸、獐芽菜苷、断氧化马钱素、连翘酯苷A、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸）的含量。方法采用Agilent Zorbax SB C18色谱柱（250 mm×4.6 mm,5μm）,流动相为0.1%甲酸水溶液-乙腈,梯度洗脱,流速为1.0 mL·min^-1,新绿原酸、绿原酸、隐绿原酸、连翘酯苷A、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸的检测波长为325 nm,獐芽菜苷、断氧化马钱素为240 nm,柱温30℃。结果 68 min分析时间内9个成分分离度良好,在各自的检测范围内浓度与峰面积呈良好的线性关系,r均〉0.9995,加样回收率为95.7%-100.6%,RSD均≤2.1%。应用所建立的方法同时测定了2个厂家生产的小儿清解颗粒中各成分的含量,其结果有一定差异。结论该法简便可行,结果可靠,可为本制剂多指标成分的质量控制提供参考方法。Objective To establish an HPLC-DAD method for the simultaneous determination of 9 components（neochlorogenic acid, chlorogenic acid, cryptchlorogenic acid, sweroside, secoxyloganin, forsythoside A, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid） in Xiaoer Qingjie granules. MethodsSamples were performed on an Agilent Zorbax SB C18 analytical column（250 mm×4.6 mm, 5 μm） with a gradient elution of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL · min^- 1. The DAD detector wavelength was 325 nm for neochlorogenic acid, chlorogenic acid, cryptchlorogenic acid, forsythoside A, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid, and 240 nm for sweroside and secoxyloganin. The column temperature was 30 ℃. Results The 9 components were well separated with good linearity between the concentration of each component and peak area within 68 min. The value of r was higher than 0.9995 for all analytes. The recoveries were 95.7%- 100.6%, and RSDs were less than 2.1%. The method was successful in simultaneously determining 9 components in Xiaoer Qingjie granules from 2 manufacturers with great variations in the determination results.Conclusion The method is simple and reliable which can provide reference for the quality control of multi-index components in Xiaoer Qingjie granules.

关 键 词：高效液相色谱-二极管阵列法 小儿清解颗粒 含量测定 新绿原酸 绿原酸 隐绿原酸 獐芽菜苷 断氧化马钱素 连翘酯苷A 3 4-二咖啡酰奎宁酸 3 5-二咖啡酰奎宁酸 4 5-二咖啡酰奎宁酸 

分 类 号：R917[医药卫生—药物分析学]