Atomic force microscopy analysis of orientation and bending of oligodeoxynucleotides in polypod-like structured DNA  

作　　者：Tomoki Shiomi Mengmeng Tan Natsuki Takahashi Masayuki Endo Tomoko Emura Kumi Hidaka Hiroshi Sugiyama Yuki Takahashi Yoshinobu Takakura Makiya Nishikawa 

机构地区：[1]Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan [2]Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan [3]Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan

出　　处：《Nano Research》2015年第12期3764-3771,共8页纳米研究（英文版）

摘　　要：We previously demonstrated that polypod-like structured DNA, or polypodna, constructed with three or more oligodeoxynucleotides （ODNs）, is efficiently taken up by immune cells such as dendritic cells and macrophages, depending on its structural complexity. The ODNs comprising the polypodna should bend to form the polypod-like structure, and may do so by adopting either a bend- type conformation or a cross-type conformation. Here, we tried to elucidate the orientation and bending of ODNs in polypodnas using atomic force microscopy （AFM）. We designed two types of pentapodnas （i.e., a polypodna with five pods） using 60- to 88-base ODNs, which were then immobilized on DNA origami frames. AFM imaging showed that the ODNs in the pentapodna adopted bend-type conformations. Tetrapodna and hexapodna also adopted bend-type conformations when they were immobilized on frames under unconstrained conditions. These findings provide useful information toward the coherent design of, and the structure-activity relationships for, a variety of DNA nanostructures.我们以前证明象多足一样组织了 DNA，或 polypodna，与三或更构造了 oligodeoxynucleotides (ODN ) ，被象树枝状的房间和巨噬细胞那样的有免疫力的房间高效地收起，取决于它的结构的复杂性。包括 polypodna 的 ODN 应该弯曲形成象多足一样结构，并且可以那么由采用 bendtype 符合构造或跨类型的符合构造做。这里，我们试着阐明取向并且用原子力量显微镜学(AFM ) 在 polypodnas ODN 弯曲。我们设计了 pentapodnas 的二种类型(即，有五个豆荚的 polypodna ) 使用 60- 到 88 底 ODN，它然后在 DNA origami 上被使不能调动装裱。AFM 成像证明在 pentapodna 的 ODN 采用了拐弯类型符合构造。当他们在非强迫的条件下面在框架上被使不能调动时， Tetrapodna 和 hexapodna 也采用了拐弯类型符合构造。这些调查结果提供向协调设计的有用信息，和 structureactivity 关系为，许多 DNA nanostructures。

关 键 词：DNA nanostructure atomic force microscopy self-asse nanotechology structure-activity relationship 

分 类 号：Q524[生物学—生物化学] O484.1[理学—固体物理]