Rho激酶在房颤患者左心耳中的表达  

Expression of Rho associated coil forming protein kinase in left atrial appendage of patients with atrial fibrillation

作　　者：苏芳菊[1] 陈永清[1] 李涛[1] 郭文昀[1] 杨红宁[1] 赵红斌[2] 

机构地区：[1]兰州军区兰州总医院心血管内科,甘肃兰州730000 [2]兰州军区兰州总医院骨研所,甘肃兰州730000

出　　处：《临床和实验医学杂志》2015年第23期1939-1941,共3页Journal of Clinical and Experimental Medicine

基　　金：甘肃省自然科学基金资助项目(编号:1308RJZA135)

摘　　要：目的研究Rho激酶(ROCK-1)及其底物—肌球蛋白磷酸酶靶亚基1(MYPT-1)在房颤患者左心耳中的表达,探讨Rock信号转导通路在房颤发生中的作用。方法 40例接受开胸手术者分为房颤组和窦性心律组,手术中取约100 mg左心耳组织。采用免疫组化ABC法检测两组左心耳组织的蛋白表达水平。结果 ROCK-1和MYPT-1在房颤患者左心耳中表达较对照组明显上调,差异有统计学意义(P<0.01)。房颤组ROCK-1和MYPT-1的蛋白表达,二者呈显著正相关(r=0.981,P<0.01)。结论房颤患者左心耳组织中明显存在ROCK-1表达上调与功能活化,提示Rho激酶信号转导通路在房颤病变的发生机制中具有重要作用。Objective To detect the expressions of Rho associated coil forming protein kinase - 1 （ ROCK - 1 ） and its substrate myosin phosphatase target subunit - 1 （ MYPT - 1 ） in left atrial appendage of patients with atrial fibrillation （AF）. Methods Left atrial appendage tis- sue samples of 40 patients undergoing cardiac surgery were examined. 20 patients had AF, 20 patients had no history of AF. The expressions of ROCK - 1 and MYPT - 1 in left atrial appendage were detected by immunohistochemistry. Results Immunohistochemistry showed that the expres- sions of ROCK - 1 and MYPT - 1 were significantly up - regulated in left atrial appendage of AF patients （ P 〈0. O1 ）. The two indicators were positively correlated （ r =0. 981, P 〈 0.01 ）. Conclusion The ROCK - 1 expression in left atrial appendage of patients with AF is up - regula- ted and activated, which implies that ROCK may play a significant role in the pathological changes in AF.

关 键 词：房颤 左心耳 ROCK-1 MYPT-1 

分 类 号：R541.75[医药卫生—心血管疾病]