Maize orthologs of rice GS5 and their transregulator are associated with kernel development  

作　　者：Jie Liu Min Deng Huan Guo Sharif Raihan Jingyun Luo Yuancheng Xu Xiaofei Dong Jianbing Yan 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University

出　　处：《Journal of Integrative Plant Biology》2015年第11期943-953,共11页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31222041);the National Hi-Tech Research and Development Program of China (2012AA10A307)

摘　　要：Genome information from model species such as rice can assist in the cloning of genes in a complex genome, such as maize. Here, we identified a maize ortholog of rice GS5 that contributes to kernel development in maize. The genome- wide association analysis of the expression levels of ZmGSs, and 15 of its 26 paralogs, identified a trans-regulator on chromosome 7, which was a BAK1-1ike gene. This gene that we named as ZmBAK1-7 could regulate the expression of ZmGS5 and three of the paralogs. Candidate-gene association analyses revealed that these five genes were associated with maize kernel development-related traits. Linkage analyses also detected that ZINGS5 and ZmI3AK1-7 co-localized with mapped QTLs. A transgenic analysis of ZINGS5 in Arabidopsis thaliana L. showed a significant increase in seed weight and cell number, suggesting that 2mG55 may have a conserved function among different plant species that affects seed development.Genome information from model species such as rice can assist in the cloning of genes in a complex genome, such as maize. Here, we identified a maize ortholog of rice GS5 that contributes to kernel development in maize. The genome- wide association analysis of the expression levels of ZmGSs, and 15 of its 26 paralogs, identified a trans-regulator on chromosome 7, which was a BAK1-1ike gene. This gene that we named as ZmBAK1-7 could regulate the expression of ZmGS5 and three of the paralogs. Candidate-gene association analyses revealed that these five genes were associated with maize kernel development-related traits. Linkage analyses also detected that ZINGS5 and ZmI3AK1-7 co-localized with mapped QTLs. A transgenic analysis of ZINGS5 in Arabidopsis thaliana L. showed a significant increase in seed weight and cell number, suggesting that 2mG55 may have a conserved function among different plant species that affects seed development.

关 键 词：Association analysis kernel development MAIZE ZmBAK1-7 ZmGS5 

分 类 号：Q943.2[生物学—植物学]