HPLC测定马先蒿属植物中的桃叶珊瑚苷  

Determination of aucubin in medicinal plant Pedicularis by HPLC

作　　者：李茂星[1,2] 曹馨元[1,2] 陶锐[1,2] 曹文龙 栾飞[1] 刘延彤[1] 

机构地区：[1]兰州军区兰州总医院药剂科全军高原环境损伤防治重点实验室,甘肃兰州730050 [2]兰州大学药学院,甘肃兰州730000 [3]天祝县藏医药开发研究所,甘肃武威733299

出　　处：《华西药学杂志》2015年第6期718-719,共2页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81374019);甘肃省中医药管理局科研立项(GZK-2015-59)

摘　　要：目的采用HPLC法测定马先蒿属植物中桃叶珊瑚苷的含量。方法色谱柱为Waters C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(10∶90),检测波长204 nm,流速1 m L·min-1。结果各样品中均能检测出桃叶珊瑚苷,但含量差异较大。结论桃叶珊瑚苷普遍存在于马先蒿属植物中;所用方法简便、准确,可用于马先蒿属药材的质量控制。OBJECTIVE To establish an HPLC method for the determination of aucubin in medicinal plant Pedicularis. METHODS Waters Cl8 column（150 mm×4.6 mm,5μm） was used, and the mobile phase was MeOH - H20（ 10 ： 90） with the detection wave- length of 204 nm and the flow rate of 1 mL·min- i. RESULTS Aucubin could be determined in all samples and there was an obvious difference in content. CONCLUSION Aucubin is widely distributed in Pedicularis. This method is simple and accurate, and can be used for the quality control of Pedicularis plants.

关 键 词：马先蒿 桃叶珊瑚苷 高效液相色谱法 含量测定 质量控制 环烯醚萜苷 

分 类 号：R917[医药卫生—药物分析学]