5-aza-cdR对神经细胞NG108-15细胞周期的影响  

The effect of 5-aza-2'-deoxycytidine on the cell cycle of NG108-15 cells

作　　者：张柱霞 孙明英[1] 杨洁[1] 姜树原[1] 刘友[1] 闫少春[1] 邵国[1,2] 

机构地区：[1]包头医学院中心实验室生物医学研究中心,包头014010 [2]首都医科大学附属宣武医院低氧适应转化医学北京市重点实验室,北京100053

出　　处：《神经解剖学杂志》2015年第6期693-697,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81060212;81160244;81360316;81460283);内蒙古自然科学基金(2010BS1104;2014MS0810);中国博士后基金(20080430851);内蒙古自治区高等学校科研项目(NJZY12221;NJZY12225);内蒙古自治区高等学校青年科技英才支持计划(NJYT-13-A10);研究生科研创新项目(S201410127)

摘　　要：目的:观察DNA甲基转移酶(DNA methyltransferase,DNMT)抑制剂5-氮-2'脱氧胞苷(5-aza-2'deoxycytidine,5-aza-cdR)对神经细胞系NG108-15细胞周期的作用,并探讨相关的机制。方法:NG108-15细胞用10μmol/L 5-aza-cdR处理,RTCA分析细胞指数,流式细胞仪检测细胞周期和细胞凋亡,8-PCR检测DNMTs及细胞周期相关基因CCND1和CCND2 mRNA的表达。结果:5-aza-cdR能抑制NG108-15细胞的增殖,5-aza-cdR处理细胞后使细胞周期发生变化,同时使DNMT1的表达降低(P<0.05)而DNMT3A和DNMT3B mRNA表达不变,并且CCND1和CCND2 mRNA表达也不变。结论:10μmol/L 5-aza-cdR可以抑制NG08-15细胞的增殖,其抑制作用可能是通过降低DNMT1表达实现的,这种抑制作用不影响CCND1和CCND2的表达。Objective：The aim of this study was to explore the effect of 5-aza-2＇-deoxycytidine（5-aza-cdR） on mouse nerval cell line NG108-15.Methods：10 μmol/L 5-aza-cdR was added to cell culture medium.The proliferation of NG108-15 was measured using the RTAC.The cell cycle and cell apoptosis were analyzed by flow cytometry analysis.The mRNA levels of DNMTs,CCND1 and CCND2 were analyzed by QPCR.Results：Normal cell cycle was interrupted by10 μmol/L 5-aza-cdR which led to inhibiting the growth of NG108-15 cell line.Cell cycles were arrested and DNMT1 mRNA was down-regulated by 10 μmol/L5-aza-cdR.Conclusion：5-aza-cdR could inhibit the proliferation of mouse nerval cell line NG108-15 cells by decreasing the expression of DNMT1 to interrupt the normal cell cycle,and this inhabition does not related to CCND1 and CCND2 mRNA.

关 键 词：NG108-15细胞 5-氮-2'脱氧胞苷 细胞周期 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]