理气化痰降浊法经验方改善AD转基因小鼠学习记忆能力  

Effects of prescriptions of Liqihuatanjiangzhuo on the learning and memory abilities of transgenic mice with AD

作　　者：王申[1] 吴芹[1] 单昊[1] 沈跃男 曹娜[1] 宋建平[1] 

机构地区：[1]盐城卫生职业技术学院医学技术学院,盐城224005

出　　处：《神经解剖学杂志》2015年第6期746-750,共5页Chinese Journal of Neuroanatomy

基　　金：2012年江苏省教育厅大学生实践创新课题(2012JSSPITP4224);2011年盐城卫生职业技术学院青年基金专项(20114103)

摘　　要：目的:通过两种不同中药方剂温脾补肾法(方剂1)和理气化痰降浊法(方剂2)经验方对阿尔茨海默病(AD)APP/PS1转基因小鼠学习记忆能力影响的对照研究探讨两首中药方剂治疗AD的可能性。方法:24只AD小鼠随机分为对照组、方剂1组、方剂2组。中药方剂(生理盐水)灌胃前后使用Morris水迷宫分别行定位航行和空间搜索试验。结果:随着时间延长,三组实验小鼠的潜伏期均呈缩短趋势,但是仅有方剂2组用药前后的潜伏期时长具有显著性差异,并比对照组、方剂1组明显缩短。用药后,方剂2组小鼠游泳总距离缩短、平均速度变慢、在站台所在象限停留时间延长、穿越站台所在位置次数明显增多,均具有统计学意义。结论:方剂2理气化痰降浊法经验方具有改善AD转基因小鼠学习记忆能力的作用。Objective：This study tries to probe the possible treatment of Alzheimer s Disease（AD） with two traditional Chinese medicine（TCM） prescriptions-Weipibushen and Liqihuatanjiangzhuo,by comparing the effects of the two herbals on the learning and memory abilities of APP/PS1 transgenic mice with AD.Methods：Twenty-four mice with AD were randomly divided into 3 groups：control group,prescription 1 group,and prescription 2 group.Before and after treatment with the two prescriptions,all the mice were tested with Morris Water Maze（MWM） for navigation and spatial search.Results：The escape latency reduced over time in all the groups,with prescription 2 group showed significant differences and its escape latency was shorter than those of control group and prescription 1 group.After treatment,significant changes were observed in the mice of prescription 2 group,with shorter swimming distance and lower speed,longer staying duration within the platform quadrants,and increased times of crossing the platform.Conclusion：TCM prescription of Liqihuatanjiangzhuo can improve the learning and memory abilities of transgenic mice with AD.

关 键 词：中药 阿尔茨海默病 转基因 MORRIS水迷宫 小鼠 

分 类 号：R285.5[医药卫生—中药学]