鞘内注射P2X7受体拮抗剂BBG对大鼠行为学和脊髓PLA2表达的影响  

Effect of intrathecal injection of P2X7R antagonist BBG on behavior and PLA2 expression in rats model

作　　者：彭志锋[1] 马国英[1] 刘颖[1] 李晨旭[1] 

机构地区：[1]山西大同大学医学院生理学教研室,大同037009

出　　处：《神经解剖学杂志》2015年第6期788-792,共5页Chinese Journal of Neuroanatomy

基　　金：山西大同大学博士科研启动经费

摘　　要：目的：探讨鞘内注射P2X7受体拮抗剂考马斯亮蓝（BBG）在福尔马林模型大鼠中的镇痛作用及对脊髓磷脂酶A2（PLA2）表达的影响。方法：雄性Wistar大鼠鞘内置管成功后鞘内注射P2X7受体拮抗剂考马斯亮蓝（BBG）后所有的大鼠左侧后肢爪掌背侧皮下注射福尔马林（5%,50μl）,观察大鼠在第一相（0~10 min）和第二相（10～60 min）的疼痛行为学比,变化（自发缩足次数）。免疫组织化学方法检测脊髓背角PLA2蛋白的表达与变化。结果：各组间大鼠第一相相比自发缩足次数没有明显的差异;第二相与对照组相比鞘内1μg、5μg及腹腔10μg BBG注射组相比,鞘内10μg BBG注射组大鼠的疼痛行为学明显减少（自发缩足次数,P〈0.05）。与此同时,鞘内10μg BBG注射组大鼠脊髓背角浅层内PLA_2免疫阳性产物的表达与其它组比较明显减少（P〈0.05）。结论：鞘内注射10μg BBG可减轻福尔马林诱导的炎性痛觉过敏,可能是通过抑制脊髓背角浅层PLA2的表达而发挥作用。Objective：To investigate the effect of intrathecal P2X7 R antagonist on analgesia and phospholipase A2（PLA2） expression in the spinal cord in a rat formalin model.Methods：Male Wistar rats,after intrathecal injection of BBG,rats were injected with formalin（5%,50 μL） on the left hind paw.Changes in pain behavior of rats in phaseⅠ（0-10 min） and phase Ⅱ（10- 60 min） were observed.Expression of PLA2 and change in dorsal horn of spinal cord was detected by immunohistochemical method.Results：There were no differences in pain response during phase Ⅰ among the groups.The phase Ⅱ pain response in 10 μg of intrathecal P2X7 R antagonist group decreased as compared to control,1 μg of intrathecal P2X7 R antagonist,5 μg of intrathecal P2X7 R antagonist,and 10 μg of systemic P2X7 R antagonist groups（P〈 0.05）.The expression of PLA2 decreased in superficial laminae in 10 μg of the intrathecal P2X7 R antagonist group compared with the ones in the control group（P〈 0.05）.Conclusion：The 10 μg of intrathecal P2X7 R antagonist BBG showed an attenuating effect on the peripheral inflammatory tissue injury induced hyperalgesia and this effect might be mediated through the inhibition of intraspinal PLA2 expression in the superficial laminae of the spinal cord.

关 键 词：磷脂酶A2 P2X7受体拮抗剂 鞘内注射 福尔马林模型 大鼠 

分 类 号：R965[医药卫生—药理学]