对碱性彗星试验主要影响因素的探讨  

Main influence factors on alkaline comet assay

作　　者：韩天娇 周长慧 常艳 

机构地区：[1]中国医药工业研究总院国家上海新药安全评价研究中心,上海201203

出　　处：《中国新药杂志》2015年第23期2664-2668,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2012ZX09302002)

摘　　要：彗星试验在化合物遗传毒性评价中的应用日益广泛,ICH S2(R1)已将肝脏彗星试验列为第2个组织/终点的体内试验;经济合作与发展组织(Organization for Economic Co-operation and Development,OECD)体内哺乳动物碱性彗星试验的指导原则(TG489)也已颁布,但彗星试验的具体操作程序各异,需要严格控制的影响因素较多,且因组织差异性,国际上无统一的标准试验方案。毒理工作者们对该试验方法的不同影响因素进行研究,以望能够形成标准的试验方案,在不影响灵敏度和特异性的前提下,提高彗星试验的可操作性,推动彗星试验在评价化合物遗传毒性方面更广泛的应用。本文主要对彗星试验的取样、制片、解旋和电泳等过程中关键的影响因素进行探讨总结,为开展和验证彗星试验方法的实验室提供参考。Comet assay is widely used in the genotoxicity safety assessments,and is listed as the second in vivo endpoint by ICH S2（ R1）. Organization for Economic Co-operation and Development（ OECD） has issued the TG489 guidline for the testing of chemicals in vivo mammalian alkaline comet assay. However,there is no standard protocol since there are many different comet assay procedures,many influences to the assay and some tissues variation. In order to standardize the comet assay protocol and to improve the operability and extensive use of the comet assay without affecting the sensitivity and specificity,toxicology workers make efforts to study the main influence on alkaline comet assay. The main influences,such as sampling,slides preparation,unwinding and electrophoresis of comet assay,were summarized in this review to make a reference for conducting and validating the comet assay.

关 键 词：碱性彗星试验 肝脏彗星试验 体外彗星试验 影响因素 

分 类 号：R965[医药卫生—药理学] R99[医药卫生—药学]